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Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818).
Ahmed, Hoda Al; Ticar, Bernadeth F; Black, Ian; Mahdi, Fakhri; Shami, Anter A; Misra, Sandeep K; Heiss, Christian; Paris, Jason J; Sharp, Joshua S; Azadi, Parastoo; Pomin, Vitor H.
Afiliación
  • Ahmed HA; Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, Mississippi, MS, USA.
  • Ticar BF; Natural Science Department, College of Arts and Sciences, Iloilo Science and Technology University, Iloilo, Philippines.
  • Black I; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
  • Mahdi F; Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, Mississippi, MS, USA.
  • Shami AA; Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, Mississippi, MS, USA.
  • Misra SK; Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, Mississippi, MS, USA.
  • Heiss C; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
  • Paris JJ; Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, Mississippi, MS, USA.
  • Sharp JS; Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, Mississippi, MS, USA.
  • Azadi P; Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Mississippi, MS, USA.
  • Pomin VH; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA.
Glycoconj J ; 40(1): 33-46, 2023 02.
Article en En | MEDLINE | ID: mdl-36454453
Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D 1H, 1H-1H COSY, 1H-1H TOCSY, 1H-1H NOESY, 1H-13C HSQC and 1H-13C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina-derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H2O2- and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H2O2- and LPS-induced ROS, and potent cell proliferative activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bivalvos / Glucanos Límite: Animals Idioma: En Revista: Glycoconj J Asunto de la revista: BIOQUIMICA / METABOLISMO Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bivalvos / Glucanos Límite: Animals Idioma: En Revista: Glycoconj J Asunto de la revista: BIOQUIMICA / METABOLISMO Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos