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Molecular Rotational Correlation Times and Nanoviscosity Determined by 111m Cd Perturbed Angular Correlation (PAC) of γ-rays Spectroscopy.
Fromsejer, Rasmus; Jensen, Marianne L; Zacate, Matthew O; Karner, Victoria L; Pecoraro, Vincent L; Hemmingsen, Lars.
Afiliación
  • Fromsejer R; Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, København, Denmark.
  • Jensen ML; Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, 2100, København, Denmark.
  • Zacate MO; Department of Physics, Geology and Engineering Technology, Northern Kentucky University, Highland Heights, KY 41099-1900, USA.
  • Karner VL; TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2 A3, Canada.
  • Pecoraro VL; Department of Chemistry, Willard H. Dow Laboratories, University of Michigan, 930N. University Ave., Ann Arbor, MI 48109-1055, USA.
  • Hemmingsen L; Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, København, Denmark.
Chemistry ; 29(9): e202203084, 2023 Feb 10.
Article en En | MEDLINE | ID: mdl-36453728
The nanoviscosity experienced by molecules in solution may be determined through measurement of the molecular rotational correlation time, τc , for example, by fluorescence and NMR spectroscopy. With this work, we apply PAC spectroscopy to determine the rate of rotational diffusion, λ=1/τc , of a de novo designed protein, TRIL12AL16C, in solutions with viscosities, ξ, from 1.7 to 88 mPa⋅s. TRIL12AL16C was selected as molecular probe because it exhibits minimal effects due to intramolecular dynamics and static line broadening, allowing for exclusive elucidation of molecular rotational diffusion. Diffusion rates determined by PAC data agree well with literature data from fluorescence and NMR spectroscopy, and scales linearly with 1/ξ in agreement with the Stokes-Einstein-Debye model. PAC experiments require only trace amounts (∼1011 ) of probe nuclei and can be conducted over a broad range of sample temperatures and pressures. Moreover, most materials are relatively transparent to γ-rays. Thus, PAC spectroscopy could find applications under circumstances where conventional techniques cannot be applied, spanning from the physics of liquids to in-vivo biochemistry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chemistry Asunto de la revista: QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Alemania
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chemistry Asunto de la revista: QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Alemania