MicroRNA-708 suppresses the proliferation, migration, and invasion of human retinoblastoma cells by targeting RAP2B, a member of the RAS oncogene family.

Dai, Changming; Yu, Huiming; Bai, Qing; Huang, Darui; Li, Jianchang; Wang, Wenqi

Dai, Changming; Yu, Huiming; Bai, Qing; Huang, Darui; Li, Jianchang; Wang, Wenqi.

Afiliación

Dai C; Department of Ophthalmology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, China.
Yu H; Department of Stomatology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, China.
Bai Q; Department of Ophthalmology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, China.
Huang D; Department of Ophthalmology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, China.
Li J; Department of Ophthalmology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, China.
Wang W; Department of Ophthalmology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, China.

Acta Biochim Pol ; 69(4): 719-724, 2022 Nov 29.

Article en En | MEDLINE | ID: mdl-36444911

RESUMEN

Retinoblastoma generally affects children and causes permanent vision failure or even death. MicroRNAs (miRs) have recently gained much attention during recent years. The miR-708 acts as a tumor suppressor in several human cancers, but the former has not been functionally characterized in human retinoblastoma. The present study was designed to investigate the role of miR-708 in human retinoblastoma. The results showed that miR-708 is significantly (P<0.05) downregulated in retinoblastoma cell lines. MiR-708 overexpression significantly (P<0.05) inhibited retinoblastoma cell growth and proliferation by inducing apoptosis. Furthermore, retinoblastoma cells overexpressing miR-708 exhibited a markedly lower migratory rate and invasiveness compared to negative control cells. The bioinformatics and dual luciferase assay revealed a RAS oncogene family protein, RAP2B, which acts as the regulatory target and functional mediator of the molecular role of miR-708 in retinoblastoma. Together, the present study revealed the tumor suppressor role of miR-708 and pointed to the therapeutic implications of miR-708/RAP2B in the treatment of retinoblastoma.

Asunto(s)

MicroARNs; Neoplasias de la Retina; Retinoblastoma; Proteínas de Unión al GTP rap; Niño; Humanos; Apoptosis/genética; Línea Celular Tumoral; Movimiento Celular/genética; Proliferación Celular/genética; Regulación Neoplásica de la Expresión Génica; Genes ras; MicroARNs/metabolismo; Invasividad Neoplásica/genética; Proteínas de Unión al GTP rap/genética; Proteínas de Unión al GTP rap/metabolismo; Neoplasias de la Retina/genética; Neoplasias de la Retina/patología; Retinoblastoma/genética; Retinoblastoma/patología

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retinoblastoma / Neoplasias de la Retina / Proteínas de Unión al GTP rap / MicroARNs Límite: Child / Humans Idioma: En Revista: Acta Biochim Pol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Polonia

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