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Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma.
Fisher, Elizaveta N; Melnikov, Evgeny S; Gegeckori, Vladimir; Potoldykova, Natalya V; Enikeev, Dmitry V; Pavlenko, Kirill A; Agatonovic-Kustrin, Snezana; Morton, David W; Ramenskaya, Galina V.
Afiliación
  • Fisher EN; I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia.
  • Melnikov ES; LLC «CPHA¼, 20/3, Nauchny Proezd, 117246 Moscow, Russia.
  • Gegeckori V; I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia.
  • Potoldykova NV; Clinical Hospital. I. V. Davidovsky, Department of Health of the City of Moscow, 11, Yauzskaya Street, 119027 Moscow, Russia.
  • Enikeev DV; I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia.
  • Pavlenko KA; Institute for Urology and Reproductive Health, I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia.
  • Agatonovic-Kustrin S; Institute for Urology and Reproductive Health, I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia.
  • Morton DW; Moscow Clinical Scientific Center, 86, Shosse Enthuziastov, 111123 Moscow, Russia.
  • Ramenskaya GV; I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia.
Molecules ; 27(22)2022 Nov 14.
Article en En | MEDLINE | ID: mdl-36431933
A novel HPLC-ESI-MS/MS method for simultaneous gonadotropin-releasing hormone (GnRH) analogs and somatostatin analog quantitation was developed and validated. The developed method was successfully applied to pharmacokinetic studies. The sample preparation process included solid-phase extraction (SPE). Effective chromatographic separation of the analytes and internal standard (dalargin) was achieved with a C18 column, using a gradient elution with two mobile phases: 0.1% v/v formic acid (aqueous solution) and 0.1% v/v formic acid (acetonitrile solution). The linearity of the method was demonstrated within a concentration range of 0.5-20 ng/mL, with correlation coefficients between 0.998-0.999 for goserelin, buserelin, triptorelin, and octreotide, respectively. The relative standard deviation (RSD, %) values for method accuracy and precision did not exceed 20% at the lower level of quantitation (LLOQ) or 15% at other concentration levels.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza