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High-resolution ion mobility spectrometry-mass spectrometry for isomeric separation of prostanoids after Girard's reagent T derivatization.
Lamont, Lieke; Hadavi, Darya; Bowman, Andrew P; Flinders, Bryn; Cooper-Shepherd, Dale; Palmer, Martin; Jordens, Jan; Mengerink, Ynze; Honing, Maarten; Langridge, James; Porta Siegel, Tiffany; Vreeken, Rob J; Heeren, Ron M A.
Afiliación
  • Lamont L; Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands.
  • Hadavi D; Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands.
  • Bowman AP; Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands.
  • Flinders B; Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands.
  • Cooper-Shepherd D; Waters Corporation, Wilmslow, UK.
  • Palmer M; Waters Corporation, Wilmslow, UK.
  • Jordens J; DSM Materials Science Center, Geleen, MD, The Netherlands.
  • Mengerink Y; DSM Materials Science Center, Geleen, MD, The Netherlands.
  • Honing M; Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands.
  • Langridge J; Waters Corporation, Wilmslow, UK.
  • Porta Siegel T; Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands.
  • Vreeken RJ; Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, The Netherlands.
  • Heeren RMA; Janssen R&D, Beerse, Belgium.
Rapid Commun Mass Spectrom ; 37(5): e9439, 2023 Mar 15.
Article en En | MEDLINE | ID: mdl-36415963
RATIONALE: Isomeric separation of prostanoids is often a challenge and requires chromatography and time-consuming sample preparation. Multiple prostanoid isomers have distinct in vivo functions crucial for understanding the inflammation process, including prostaglandins E2 (PGE2 ) and D2 (PGD2 ). High-resolution ion mobility spectrometry (IMS) based on linear ion transport in low-to-moderate electric fields and nonlinear ion transport in strong electric fields emerges as a broad approach for rapid separations prior to mass spectrometry. METHODS: Derivatization with Girard's reagent T (GT) was used to overcome inefficient ionization of prostanoids in negative ionization mode due to poor deprotonation of the carboxylic acid group. Three high-resolution IMS techniques, namely linear cyclic IMS, linear trapped IMS, and nonlinear high-field asymmetric waveform IMS, were compared for the isomeric separation and endogenous detection of prostanoids present in intestinal tissue. RESULTS: Direct infusion of GT-derivatized prostanoids proved to increase the ionization efficiency in positive ionization mode by a factor of >10, which enabled detection of these molecules in endogenous concentration levels. The high-resolution IMS comparison revealed its potential for rapid isomeric analysis of biologically relevant prostanoids. Strengths and weaknesses of both linear and nonlinear IMS are discussed. Endogenous prostanoid detection in intestinal tissue extracts demonstrated the applicability of our approach in biomedical research. CONCLUSIONS: The applied derivatization strategy offers high sensitivity and improved stereoisomeric separation for screening of complex biological systems. The high-resolution IMS comparison indicated that the best sensitivity and resolution are achieved by linear and nonlinear IMS, respectively.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Prostaglandinas / Espectrometría de Movilidad Iónica Idioma: En Revista: Rapid Commun Mass Spectrom Año: 2023 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Prostaglandinas / Espectrometría de Movilidad Iónica Idioma: En Revista: Rapid Commun Mass Spectrom Año: 2023 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido