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The Dietary Isothiocyanate Erucin Reduces Kidney Cell Motility by Disturbing Tubulin Polymerization.
Guerreiro, Íris; Vidovic, Bojana; Costa, João G; Martins, Marta; Ferreira, Sandra; Oliveira, Nuno G; Saraiva, Nuno; Fernandes, Ana S.
Afiliación
  • Guerreiro Í; CBIOS, Universidade Lusófona's Research Center for Biosciences & Health Technologies, Lisbon, 1749-024, Portugal.
  • Vidovic B; Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, 28805, Spain.
  • Costa JG; Faculty of Pharmacy, University of Belgrade, Belgrade, 11221, Serbia.
  • Martins M; CBIOS, Universidade Lusófona's Research Center for Biosciences & Health Technologies, Lisbon, 1749-024, Portugal.
  • Ferreira S; CBIOS, Universidade Lusófona's Research Center for Biosciences & Health Technologies, Lisbon, 1749-024, Portugal.
  • Oliveira NG; Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, 28805, Spain.
  • Saraiva N; CBIOS, Universidade Lusófona's Research Center for Biosciences & Health Technologies, Lisbon, 1749-024, Portugal.
  • Fernandes AS; Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, 28805, Spain.
Mol Nutr Food Res ; 67(3): e2200581, 2023 02.
Article en En | MEDLINE | ID: mdl-36415106
SCOPE: Epidemiological evidence associates the consumption of cruciferous vegetables with reduced risk of several cancers, including renal cell carcinoma. Erucin can be generated by in vivo reduction of sulforaphane or by enzymatic hydrolysis of glucoerucin. Contrarily to sulforaphane, only limited studies have addressed the anticancer properties of erucin. This study aims at evaluating the impact of erucin on renal cell biology. METHODS AND RESULTS: The effects of erucin were assessed in 786-O and Vero-E6 cells, representative of human renal cancer and non- cancer kidney cells, respectively. Erucin induced a concentration-dependent decrease in cell viability and cell cycle arrest at G2/Mitosis. In Vero-E6 cells erucin modestly reduced intracellular reactive oxygen species levels while in 786-O no effects were detected. After erucin treatment, both cell lines revealed altered morphology, with a concentration-dependent change from an elongated shape towards a smaller round conformation. Moreover, erucin affected cell adhesion and strongly altered the tubulin network structure and specifically microtubule polymerization. These results are in line with the observed decrease in collective and single cell migration and G2/Mitosis arrest. CONCLUSIONS: Overall, erucin may have a beneficial impact in reducing the motility of renal cancer cells. Our results contribute to explore possible dietary approaches for secondary/tertiary renal cancer chemoprevention.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Neoplasias Renales Límite: Humans Idioma: En Revista: Mol Nutr Food Res Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2023 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Neoplasias Renales Límite: Humans Idioma: En Revista: Mol Nutr Food Res Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2023 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Alemania