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Three-dimensional biomimetic reinforced chitosan/gelatin composite scaffolds containing PLA nano/microfibers for soft tissue engineering application.
Eftekhari-Pournigjeh, Fatemeh; Saeed, Mahdi; Rajabi, Sarah; Tamimi, Maryam; Pezeshki-Modaress, Mohamad.
Afiliación
  • Eftekhari-Pournigjeh F; Department of Biomedical Engineering, Islamic Azad University, Central Tehran Branch, Tehran, Iran; Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
  • Saeed M; Department of Biomedical Engineering, Islamic Azad University, Central Tehran Branch, Tehran, Iran; Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Islamic Azad University, Central Tehran Branch, Tehran, Iran. Electronic address: mah.saeed@iauctb.ac.i
  • Rajabi S; Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
  • Tamimi M; Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
  • Pezeshki-Modaress M; Burn Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Plastic and Reconstructive Surgery, Hazrat Fatemeh Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address: Pezeshkimodaress.m@iums.ac.ir.
Int J Biol Macromol ; 225: 1028-1037, 2023 Jan 15.
Article en En | MEDLINE | ID: mdl-36414076
In the current study, we successfully prepared chitosan/gelatin composite scaffolds reinforced by centrifugally spun polylactic acid (PLA) chopped nano/microfibers (PLA-CFs). Herein, different amounts of PLA-CFs (0 %, 1 %, 2 %, 3 %, and 4 % w/v) dispersed in chitosan/gelatin solution were used. Morphological characterization of prepared scaffolds revealed that at the initial stage of adding PLA-CFs, the chopped fibers were localized at the wall of the pores; however, as the fiber load increased, aggregations of chopped-fibers could be seen. Also, mechanical evaluation of scaffolds in terms of compression and tensile mode showed that samples reinforced with 2 % PLA-CFs had enhanced mechanical properties. Indeed, its tensile strength increased from 123.8 to 247.2 kPa for dry and 18.9 to 48.6 kPa for wet conditions. Furthermore, the tensile modulus associated with both conditions increased from 2.99 MPa and 44.5 kPa to 6.43 MPa and 158.4 kPa, respectively. The results of cell culture studies also confirmed that the prepared composite scaffold exhibited appropriate biocompatibility, cell proliferation and migration. The cell infiltration study of the samples revealed that scaffolds reinforced with 2 % PLA-CFs had significantly better cell penetration and distribution compared with the control ones on both days (7 and 14).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos