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Evaluation of promoter hypermethylation of tumor suppressor gene BRCA1 in epithelial ovarian cancer.
Das, Jhuma; Chandra, Lal; Gandhi, Gauri; Amle, Dnyanesh B; Patnayak, Rachana L; Khurana, Nita; Saxena, Alpana.
Afiliación
  • Das J; Department of Biochemistry, Maulana Azad Medical College, New Delhi, India.
  • Chandra L; Department of Biochemistry, Maulana Azad Medical College, New Delhi, India.
  • Gandhi G; Department of Obstetrics and Gynaecology, Maulana Azad Medical College, New Delhi, India.
  • Amle DB; Department of Biochemistry, All India Institute of Medical Sciences, Nagpur, Maharashtra, India.
  • Patnayak RL; Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, India.
  • Khurana N; Department of Pathology, Maulana Azad Medical College, New Delhi, India.
  • Saxena A; Department of Biochemistry, Maulana Azad Medical College, New Delhi, India.
J Cancer Res Ther ; 18(6): 1578-1582, 2022.
Article en En | MEDLINE | ID: mdl-36412414
Context: Epithelial ovarian cancer (EOC) is a serious gynecological issue worldwide and its late detection is the major encumbrance in treatment procedures. Hypermethylation-mediated BRCA1 gene silencing results in failure of the repair system of damaged DNA playing an important role in ovarian carcinogenesis. BRCA1 gene hypermethylation can serve as a safe and highly specific clinical marker for EOC. Aims: The present study was conducted to evaluate the promoter hypermethylation of BRCA1 gene in EOC patients. Settings and Design: This hospital-based case-control study carried out in the tertiary care hospital in New Delhi. Subjects and Methods: Promoter hypermethylation of BRCA1 gene was examined in 30 EOC diagnosed untreated cases confirmed by histopathological examinations and compared with 30 normal healthy controls matched for age using methylation specific-polymerase chain reaction. Results: We found significantly higher BRCA1 promoter hypermethylation in the serum of EOC cases as compared to controls with P < 0.0001. BRCA1 gene methylation was found to have 70% sensitivity for the diagnosis of EOC with 100% specificity. A significant difference was observed in the range of CA125 levels, B12 and Folate levels between EOC cases and controls. Conclusions: We conclude that BRCA1 gene is significantly hypermethylated in EOC patients and thus can prove to be a noninvasive diagnostic tool. Our results provide prefatory evidence that epithelial ovarian epigenome can be influenced by dietary nutrients.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas Tipo de estudio: Observational_studies Límite: Female / Humans Idioma: En Revista: J Cancer Res Ther Asunto de la revista: NEOPLASIAS / TERAPEUTICA Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: India
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas Tipo de estudio: Observational_studies Límite: Female / Humans Idioma: En Revista: J Cancer Res Ther Asunto de la revista: NEOPLASIAS / TERAPEUTICA Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: India