Your browser doesn't support javascript.
loading
Composite Mesoporous Silica Nanoparticles with Dual-color Afterglow for Cross-correlation-based Living Cell Imaging.
Sun, Mingqi; Meng, Jiaqi; Bao, Weier; Liu, Ming; Li, Xiaojuan; Wang, Zicheng; Ma, Zhecheng; Wang, Xuefei; Tian, Zhiyuan.
Afiliación
  • Sun M; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Meng J; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Bao W; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Liu M; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Li X; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Wang Z; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Ma Z; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Wang X; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
  • Tian Z; School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049, China.
Chemphyschem ; 24(6): e202200716, 2023 03 14.
Article en En | MEDLINE | ID: mdl-36404675
Room temperature phosphorescence (RTP) materials are characterized with emission after removing the excitation source. Such long-lived emission feature possesses great potential in biological fluorescence imaging because it enables a way regarding temporal dimension for separating the interference of autofluorescence and common noises typically encountered in conventional fluorescence imaging. Herein, we constructed a new type of mesoporous silica nanoparticles (MSNs)-based composite nanoparticles (NPs) with dual-color long-lived emission, namely millisecond-level green phosphorescence and sub-millisecond-level delayed red fluorescence by encapsulating a typical RTP dye and Rhodamine dye in the cavities of the MSNs with the former acting as energy donor (D) while the latter as acceptor (A). Benefiting from the close D-A proximity, energy match between the donor and the acceptor and the optimized D/A ratio in the composite NPs, efficient triplet-to-singlet Förster resonance energy transfer (TS-FRET) in the NPs occurred upon exciting the donor, which enabled dual-color long-lived emission. The preliminary results of dual-color correlation imaging of live cells based on such emission feature unequivocally verified the unique ability of such NPs for distinguishing the false positive generated by common emitters with single-color emission feature.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transferencia Resonante de Energía de Fluorescencia / Nanopartículas Idioma: En Revista: Chemphyschem Asunto de la revista: BIOFISICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transferencia Resonante de Energía de Fluorescencia / Nanopartículas Idioma: En Revista: Chemphyschem Asunto de la revista: BIOFISICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania