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Evidence supporting the oncogenic role of BAZ1B in colorectal cancer.
Grochowska, Aleksandra; Statkiewicz, Malgorzata; Kulecka, Maria; Cybulska, Magdalena; Sandowska-Markiewicz, Zuzanna; Kopczynski, Michal; Drezinska-Wolek, Edyta; Tysarowski, Andrzej; Prochorec-Sobieszek, Monika; Ostrowski, Jerzy; Mikula, Michal.
Afiliación
  • Grochowska A; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Statkiewicz M; Department of Gastroenterology, Hepatology and Clinical Oncology, Centre for Postgraduate Medical Education Warsaw 01-813, Poland.
  • Kulecka M; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Cybulska M; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Sandowska-Markiewicz Z; Department of Gastroenterology, Hepatology and Clinical Oncology, Centre for Postgraduate Medical Education Warsaw 01-813, Poland.
  • Kopczynski M; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Drezinska-Wolek E; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Tysarowski A; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Prochorec-Sobieszek M; Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Ostrowski J; Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
  • Mikula M; Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie National Research Institute of Oncology Warsaw 02-781, Poland.
Am J Cancer Res ; 12(10): 4751-4763, 2022.
Article en En | MEDLINE | ID: mdl-36381331
Bromodomain Adjacent to Zinc Finger Domain 1B (BAZ1B) is involved in multiple nuclear processes, and its role in tumorigenesis is emerging. However, the function of BAZ1B in colorectal cancer (CRC) remains largely unexplored. High-density tissue microarrays comprising 100 pairs of matched normal colon and treatment-naïve CRC samples were analyzed by immunohistochemistry with an anti-BAZ1B antibody. The HCT116 and SW480 CRC cell lines were used for overexpression and small hairpin RNA-mediated BAZ1B knockdown models, respectively. Both cell lines were xenografted to immunodeficient NU/J mice to assess tumor burden. The molecular consequences of alterations of BAZ1B expression were assessed by RNA-Seq of xenografts and functional analyses using the Reactome database. Immunohistochemical analysis of BAZ1B showed that BAZ1B staining intensity was higher in 93 tumor specimens and significantly correlated with tumor size (P = 0.03), but not with the presence of KRAS mutation. BAZ1B overexpression significantly increased and its knockdown inhibited the proliferation of HCT116 and SW480 cell lines, respectively. These findings were reproduced when both cell lines were grown as xenografts. RNA-Seq of HCT116 and SW480 xenografts identified 2046 and 99 differentially expressed genes (DEGs) (adjusted P ≤ 0.05), respectively. Functional annotation of DEGs identified already established as well as new molecular processes dependent on BAZ1B protein expression. In conclusion, BAZ1B is overexpressed in CRC tissue and contributes to CRC cell proliferation in vitro and in vivo. The data support the emerging oncogenic role of BAZ1B in cancerogenesis including in CRC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Am J Cancer Res Año: 2022 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Am J Cancer Res Año: 2022 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos