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Multi-Organ Transcriptome Response of Lumpfish (Cyclopterus lumpus) to Aeromonas salmonicida Subspecies salmonicida Systemic Infection.
Chakraborty, Setu; Hossain, Ahmed; Cao, Trung; Gnanagobal, Hajarooba; Segovia, Cristopher; Hill, Stephen; Monk, Jennifer; Porter, Jillian; Boyce, Danny; Hall, Jennifer R; Bindea, Gabriela; Kumar, Surendra; Santander, Javier.
Afiliación
  • Chakraborty S; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Hossain A; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Cao T; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Gnanagobal H; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Segovia C; Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Hill S; Cold-Ocean Deep-Sea Research Facility, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Monk J; Dr. Joe Brown Aquatic Research Building, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Porter J; Dr. Joe Brown Aquatic Research Building, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Boyce D; Dr. Joe Brown Aquatic Research Building, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Hall JR; Aquatic Research Cluster, CREAIT Network, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
  • Bindea G; INSERM, Laboratory of Integrative Cancer Immunology, 75006 Paris, France.
  • Kumar S; Equipe Labellisée Ligue Contre Le Cancer, 75013 Paris, France.
  • Santander J; Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, 75006 Paris, France.
Microorganisms ; 10(11)2022 Oct 26.
Article en En | MEDLINE | ID: mdl-36363710
Lumpfish is utilized as a cleaner fish to biocontrol sealice infestations in Atlantic salmon farms. Aeromonas salmonicida, a Gram-negative facultative intracellular pathogen, is the causative agent of furunculosis in several fish species, including lumpfish. In this study, lumpfish were intraperitoneally injected with different doses of A. salmonicida to calculate the LD50. Samples of blood, head-kidney, spleen, and liver were collected at different time points to determine the infection kinetics. We determined that A. salmonicida LD50 is 102 CFU per dose. We found that the lumpfish head-kidney is the primary target organ of A. salmonicida. Triplicate biological samples were collected from head-kidney, spleen, and liver pre-infection and at 3- and 10-days post-infection for RNA-sequencing. The reference genome-guided transcriptome assembly resulted in 6246 differentially expressed genes. The de novo assembly resulted in 403,204 transcripts, which added 1307 novel genes not identified by the reference genome-guided transcriptome. Differential gene expression and gene ontology enrichment analyses suggested that A. salmonicida induces lethal infection in lumpfish by uncontrolled and detrimental blood coagulation, complement activation, inflammation, DNA damage, suppression of the adaptive immune system, and prevention of cytoskeleton formation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microorganisms Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microorganisms Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza