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Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva.
Tokuyama-Toda, Reiko; Terada-Ito, Chika; Muraoka, Masaaki; Horiuchi, Toshikatsu; Amemiya, Tsuyoshi; Fukuoka, Airi; Hamada, Yoshiki; Takebe, Yusuke; Ogawa, Takashi; Fujii, Seiko; Kikuta, Toshihiro; Sejima, Shunsuke; Satomura, Kazuhito.
Afiliación
  • Tokuyama-Toda R; Department of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.
  • Terada-Ito C; Department of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.
  • Muraoka M; Certified Non-Profit Organization Biomedical Science Association, 2-20-8, Kamiosaki, Shinagawa-ku 141-0021, Tokyo, Japan.
  • Horiuchi T; Department of Oral and Maxillofacial Surgery, Saiseikai Yokohamashi Tobu Hospital, 3-6-1, Shimosueyoshi, Tsurumi-ku, Yokohama City 230-8765, Kanagawa, Japan.
  • Amemiya T; Department of Oral and Maxillofacial Surgery, Saiseikai Yokohamashi Tobu Hospital, 3-6-1, Shimosueyoshi, Tsurumi-ku, Yokohama City 230-8765, Kanagawa, Japan.
  • Fukuoka A; Department of Oral and Maxillofacial Surgery, Saiseikai Yokohamashi Tobu Hospital, 3-6-1, Shimosueyoshi, Tsurumi-ku, Yokohama City 230-8765, Kanagawa, Japan.
  • Hamada Y; Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, 2-1-3, Tsu-rumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.
  • Takebe Y; Department of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.
  • Ogawa T; Department of Oral and Maxillofacial Surgery, Tokyo Medical University Hachioji Medical Center, 1163, Tatemachi, Hachioji City 193-0998, Tokyo, Japan.
  • Fujii S; Department of Oral and Maxillofacial Surgery, Shin-Yurigaoka General Hospital, 255, Furusawatsuko, Asao-ku, Kawasaki City 215-0026, Kanagawa, Japan.
  • Kikuta T; Department of Oral and Maxillofacial Surgery, Shin-Yurigaoka General Hospital, 255, Furusawatsuko, Asao-ku, Kawasaki City 215-0026, Kanagawa, Japan.
  • Sejima S; Certified Non-Profit Organization Biomedical Science Association, 2-20-8, Kamiosaki, Shinagawa-ku 141-0021, Tokyo, Japan.
  • Satomura K; Department of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Kanagawa, Japan.
Diagnostics (Basel) ; 12(11)2022 Oct 22.
Article en En | MEDLINE | ID: mdl-36359412
The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for specialized laboratory technicians and a long turnaround time. Therefore, we have established and reported a rapid diagnostic method using a small amount of saliva as a sample using a lightweight mobile qPCR device. This study aimed to improve the existing method and increase the detection sensitivity and specificity. The detection specificity of CDC N1 and N2 was examined by improving qPCR reagents and polymerase chain reaction conditions for the previously reported method. Furthermore, the feasibility of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA was examined using both the previous method and the improved method in patients with COVID-19. The results showed that the improved method increased the specificity and sensitivity. This improved method is useful for the rapid diagnosis of SARS-CoV-2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Diagnostics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Diagnostics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza