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Electrophoresis-Assisted Multilayer Assembly of Nanoparticles for Sensitive Lateral Flow Immunoassay.
Panferov, Vasily G; Ivanov, Nikita A; Mazzulli, Tony; Brinc, Davor; Kulasingam, Vathany; Krylov, Sergey N.
Afiliación
  • Panferov VG; Department of Chemistry, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.
  • Ivanov NA; Centre for Research on Biomolecular Interactions, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.
  • Mazzulli T; A.N. Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology" Russian Academy of Sciences, 33 Leninsky Prospect, Moscow, 119071, Russia.
  • Brinc D; Department of Chemistry, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.
  • Kulasingam V; Centre for Research on Biomolecular Interactions, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.
  • Krylov SN; Sinai Health, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada.
Angew Chem Int Ed Engl ; 62(2): e202215548, 2023 01 09.
Article en En | MEDLINE | ID: mdl-36357330
Lateral flow immunoassay (LFIA) is a rapid, simple, and inexpensive point-of-need method. A major limitation of LFIA is a high limit of detection (LOD), which impacts its diagnostic sensitivity. To overcome this limitation, we introduce a signal-enhancement procedure that is performed after completing LFIA and involves controllably moving biotin- and streptavidin-functionalized gold nanoparticles by electrophoresis. The nanoparticles link to immunocomplexes forming multilayer aggregates on the test strip, thus, enhancing the signal. Here, we demonstrate lowering the LOD of hepatitis B surface antigen from approximately 8 to 0.12 ng mL-1 , making it clinically acceptable. Testing 118 clinical samples for hepatitis B showed that signal enhancement increased the diagnostic sensitivity of LFIA from 73 % to 98 % while not affecting its 95 % specificity. Electrophoresis-driven enhancement of LFIA is universal (antigen-independent), takes two minutes, and can be performed by an untrained person.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania