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Droplet Microfluidics Enables Tracing of Target Cells at the Single-Cell Transcriptome Resolution.
Liu, Yang; Wang, Shiyu; Lyu, Menghua; Xie, Run; Guo, Weijin; He, Ying; Shi, Xuyang; Wang, Yang; Qi, Jingyu; Zhu, Qianqian; Zhang, Hui; Luo, Tao; Chen, Huaying; Zhu, Yonggang; Dong, Xuan; Li, Zida; Gu, Ying; Liu, Longqi; Xu, Xun; Liu, Ya.
Afiliación
  • Liu Y; BGI-Shenzhen, Shenzhen 518083, China.
  • Wang S; BGI-Shenzhen, Shenzhen 518083, China.
  • Lyu M; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Xie R; BGI-Shenzhen, Shenzhen 518083, China.
  • Guo W; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • He Y; Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China.
  • Shi X; Department of Biomedical Engineering, Shantou University, Shantou 515063, China.
  • Wang Y; Department of Gynaecological Oncology, Cancer Hospital Chinese Academy of Medical Sciences, Shenzhen Center, Shenzhen 518116, China.
  • Qi J; BGI-Shenzhen, Shenzhen 518083, China.
  • Zhu Q; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Zhang H; BGI-Shenzhen, Shenzhen 518083, China.
  • Luo T; BGI-Shenzhen, Shenzhen 518083, China.
  • Chen H; BGI-Shenzhen, Shenzhen 518083, China.
  • Zhu Y; BGI-Shenzhen, Shenzhen 518083, China.
  • Dong X; Department of Mechanical & Electrical Engineering, Xiamen University, Xiamen 361101, China.
  • Li Z; School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen 518055, China.
  • Gu Y; School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen 518055, China.
  • Liu L; BGI-Shenzhen, Shenzhen 518083, China.
  • Xu X; Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China.
  • Liu Y; BGI-Shenzhen, Shenzhen 518083, China.
Bioengineering (Basel) ; 9(11)2022 Nov 10.
Article en En | MEDLINE | ID: mdl-36354585
The rapid promotion of single-cell omics in various fields has begun to help solve many problems encountered in research, including precision medicine, prenatal diagnosis, and embryo development. Meanwhile, single-cell techniques are also constantly updated with increasing demand. For some specific target cells, the workflow from droplet screening to single-cell sequencing is a preferred option and should reduce the impact of operation steps, such as demulsification and cell recovery. We developed an all-in-droplet method integrating cell encapsulation, target sorting, droplet picoinjection, and single-cell transcriptome profiling on chips to achieve labor-saving monitoring of TCR-T cells. As a proof of concept, in this research, TCR-T cells were encapsulated, sorted, and performed single-cell transcriptome sequencing (scRNA-seq) by injecting reagents into droplets. It avoided the tedious operation of droplet breakage and re-encapsulation between droplet sorting and scRNA-seq. Moreover, convenient device operation will accelerate the progress of chip marketization. The strategy achieved an excellent recovery performance of single-cell transcriptome with a median gene number over 4000 and a cross-contamination rate of 8.2 ± 2%. Furthermore, this strategy allows us to develop a device with high integrability to monitor infused TCR-T cells, which will promote the development of adoptive T cell immunotherapy and their clinical application.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioengineering (Basel) Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioengineering (Basel) Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza