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Chronic inflammation decreases HSC fitness by activating the druggable Jak/Stat3 signaling pathway.
Grusanovic, Srdjan; Danek, Petr; Kuzmina, Maria; Adamcova, Miroslava K; Burocziova, Monika; Mikyskova, Romana; Vanickova, Karolina; Kosanovic, Sladjana; Pokorna, Jana; Reinis, Milan; Brdicka, Tomas; Alberich-Jorda, Meritxell.
Afiliación
  • Grusanovic S; Department of Hemato-Oncology, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Danek P; Faculty of Science, Charles University, Prague, Czech Republic.
  • Kuzmina M; Childhood Leukaemia Investigation Prague, Department of Pediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University in Prague, University Hospital Motol, Prague, Czech Republic.
  • Adamcova MK; Department of Hemato-Oncology, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Burocziova M; Department of Hemato-Oncology, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Mikyskova R; Faculty of Science, Charles University, Prague, Czech Republic.
  • Vanickova K; Department of Hemato-Oncology, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Kosanovic S; Childhood Leukaemia Investigation Prague, Department of Pediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University in Prague, University Hospital Motol, Prague, Czech Republic.
  • Pokorna J; Department of Hemato-Oncology, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Reinis M; Department of Immunological and Tumor models, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Brdicka T; Department of Hemato-Oncology, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Alberich-Jorda M; Faculty of Science, Charles University, Prague, Czech Republic.
EMBO Rep ; 24(1): e54729, 2023 01 09.
Article en En | MEDLINE | ID: mdl-36341527
Chronic inflammation represents a major threat to human health since long-term systemic inflammation is known to affect distinct tissues and organs. Recently, solid evidence demonstrated that chronic inflammation affects hematopoiesis; however, how chronic inflammation affects hematopoietic stem cells (HSCs) on the mechanistic level is poorly understood. Here, we employ a mouse model of chronic multifocal osteomyelitis (CMO) to assess the effects of a spontaneously developed inflammatory condition on HSCs. We demonstrate that hematopoietic and nonhematopoietic compartments in CMO BM contribute to HSC expansion and impair their function. Remarkably, our results suggest that the typical features of murine multifocal osteomyelitis and the HSC phenotype are mechanistically decoupled. We show that the CMO environment imprints a myeloid gene signature and imposes a pro-inflammatory profile on HSCs. We identify IL-6 and the Jak/Stat3 signaling pathway as critical mediators. However, while IL-6 and Stat3 blockage reduce HSC numbers in CMO mice, only inhibition of Stat3 activity significantly rescues their fitness. Our data emphasize the detrimental effects of chronic inflammation on stem cell function, opening new venues for treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interleucina-6 / Inflamación Límite: Animals / Humans Idioma: En Revista: EMBO Rep Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interleucina-6 / Inflamación Límite: Animals / Humans Idioma: En Revista: EMBO Rep Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido