Your browser doesn't support javascript.
loading
The effects of painless nerve growth factor on human microglia polarization.
Lisi, Lucia; Marinelli, Silvia; Ciotti, Gabriella Maria Pia; Pizzoferrato, Michela; Palmerio, Federica; Chiavari, Marta; Cattaneo, Antonino; Navarra, Pierluigi.
Afiliación
  • Lisi L; Section of Pharmacology, Department of Healthcare Surveillance and Bioethics, Catholic University Medical School, Fondazione Policlinico Universitario A. Gemelli-IRCCS, Rome, Italy.
  • Marinelli S; European Brain Research Institute-Fondazione Rita Levi Montalcini, Rome, Italy.
  • Ciotti GMP; Section of Pharmacology, Department of Healthcare Surveillance and Bioethics, Catholic University Medical School, Fondazione Policlinico Universitario A. Gemelli-IRCCS, Rome, Italy.
  • Pizzoferrato M; Section of Pharmacology, Department of Healthcare Surveillance and Bioethics, Catholic University Medical School, Fondazione Policlinico Universitario A. Gemelli-IRCCS, Rome, Italy.
  • Palmerio F; European Brain Research Institute-Fondazione Rita Levi Montalcini, Rome, Italy.
  • Chiavari M; Section of Pharmacology, Department of Healthcare Surveillance and Bioethics, Catholic University Medical School, Fondazione Policlinico Universitario A. Gemelli-IRCCS, Rome, Italy.
  • Cattaneo A; European Brain Research Institute-Fondazione Rita Levi Montalcini, Rome, Italy.
  • Navarra P; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
Front Cell Neurosci ; 16: 969058, 2022.
Article en En | MEDLINE | ID: mdl-36339818
Previous studies in the rat suggest that microglial cells represent a potential druggable target for nerve growth factor (NGF) in the brain. The painless human Nerve Growth Factor (hNGFp) is a recombinant mutated form of human nerve growth factor (hNGF) that shows identical neurotrophic and neuroprotective properties of wild-type NGF but displays at least 10-fold lower algogenic activity. From the pharmacological point of view, hNGFp is a biased tropomyosin receptor kinase A (TrkA) agonist and displays a significantly lower affinity for the p75 neurotrophin receptor (p75NTR). This study aimed to evaluate the expression of TrkA and p75NTR NGF receptors in two different human microglia cell lines, and to investigate the effects of hNGFp and wild-type NGF (NGF) on L-arginine metabolism, taken as a marker of microglia polarization. Both NGF receptors are expressed in human microglia cell lines and are effective in transducing signals triggered by NGF and hNGFp. The latter and, to a lesser extent, NGF inhibit cytokine-stimulated inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production in these cells. Conversely NGF but not hNGFp stimulates arginase-mediated urea production.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Neurosci Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Neurosci Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza