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Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application.
Sugiura, Kazunori; Nagai, Takeharu.
Afiliación
  • Sugiura K; SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka, Japan.
  • Nagai T; SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka, Japan. ng1@sanken.osaka-u.ac.jp.
Commun Biol ; 5(1): 1172, 2022 11 03.
Article en En | MEDLINE | ID: mdl-36329112
To perform correlation analysis between different physiological parameters using fluorescent protein-based functional probes, diversification of wavelength properties of fluorescent proteins is underway. However, the shortest emission wavelength of fluorescent proteins has not been updated for more than 10 years. Here, we report the development of Sumire, a fluorescent protein emitting 414 nm violet fluorescence from a hydrated chromophore. The Sumire's fluorescence property allows for the creation of FRET probes that can be used simultaneously with CFP-YFP based FRET probes for multi-parameter analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transferencia Resonante de Energía de Fluorescencia Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transferencia Resonante de Energía de Fluorescencia Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido