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Chromatin accessibility analysis reveals distinct functions for HDAC and EZH2 activities in early appendage regeneration.
Arbach, Hannah E; Harland-Dunaway, Marcus; Braden, Christopher; Chitsazan, Alexander D; Pickering, Eleanor; Patel, Jeet H; Wills, Andrea E.
Afiliación
  • Arbach HE; Department of Biochemistry, University of Washington, Seattle, Washington, USA.
  • Harland-Dunaway M; Riverside Department of Botany and Plant Sciences, University of California, La Jolla, California, USA.
  • Braden C; Department of Biochemistry, University of Washington, Seattle, Washington, USA.
  • Chitsazan AD; Oregon Health Sciences Center For Early Detection Advanced Research Center (CEDAR), Portland, Oregon, USA.
  • Pickering E; Department of Biochemistry, University of Washington, Seattle, Washington, USA.
  • Patel JH; Department of Biochemistry, University of Washington, Seattle, Washington, USA.
  • Wills AE; Program in Molecular and Cell Biology, University of Washington, Seattle, Washington, USA.
Wound Repair Regen ; 30(6): 707-725, 2022 11.
Article en En | MEDLINE | ID: mdl-36301622
Xenopus tropicalis tadpoles have the capacity for scarless regeneration of appendages including the limb and tail. Following injury, transcriptional programs must be activated and inactivated with high spatial and temporal resolution to result in a properly patterned appendage. Functional studies have established that histone-modifying enzymes that act to close chromatin are required for regeneration, but the genomic regions sensitive to these activities are not fully established. Here we show that early inhibition of HDAC or EZH2 activity results in incomplete tail regeneration. To identify how each of these perturbations impacts chromatin accessibility, we applied an assay for transposase-accessible chromatin (ATAC-seq) to HDAC or EZH2-inhibited regenerating tadpoles. We find that neither perturbation results in a global increase in chromatin accessibility, but that both inhibitors have targeted effects on chromatin accessibility and gene expression. Upon HDAC inhibition, regulatory regions neighbouring genes associated with neuronal regeneration are preferentially accessible, whereas regions associated with immune response and apoptosis are preferentially accessible following EZH2 inhibition. Together, these results suggest distinct roles for these two chromatin-closing activities in appendage regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Cromatina Límite: Animals Idioma: En Revista: Wound Repair Regen Asunto de la revista: DERMATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Cromatina Límite: Animals Idioma: En Revista: Wound Repair Regen Asunto de la revista: DERMATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos