Your browser doesn't support javascript.
loading
Immunomodulatory Effect of Polysaccharides from the Mushroom-Forming Basidiomycete Gymnopilus imperialis (Agaricomycetes, Basidiomycota).
Caldas, Lhaís A; Santos, Patricia D; Carbonero, Elaine R; Ionta, Marisa; Miyazawa, Marta; Caixeta, Ester S; Fregnan, Antonio M; Nóbrega, Bianca Barros; Di Medeiros, Maria Carolina B; Menolli, Nelson; Soares, Douglas M M; Stevani, Cassius V; Sartorelli, Patricia.
Afiliación
  • Caldas LA; Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, SP, Brazil.
  • Santos PD; Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, SP, Brazil.
  • Carbonero ER; Instituto de Química, Universidade Federal de Catalão, Catalão 75705-220, GO, Brazil.
  • Ionta M; Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas 37130-001, MG, Brazil.
  • Miyazawa M; Faculdade de Odontologia, Universidade Federal de Alfenas, Alfenas 37130-001, MG, Brazil.
  • Caixeta ES; Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas 37130-001, MG, Brazil.
  • Fregnan AM; Faculdade de Odontologia, Universidade Federal de Alfenas, Alfenas 37130-001, MG, Brazil.
  • Nóbrega BB; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo 05508-070, SP, Brazil.
  • Di Medeiros MCB; Medicine Departament, University Center of Planalto Central Aparecido dos Santos, Brasilia 72445-020, DF, Brazil.
  • Menolli N; IFungiLab, Subárea de Biologia (SAB), Departamento de Ciências da Natureza e Matemática (DCM), Campus São Paulo (SPO), Instituto Federal de Educação, Ciência e Tecnologia de São Paulo, São Paulo 01109-010, SP, Brazil.
  • Soares DMM; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo 05508-070, SP, Brazil.
  • Stevani CV; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo 05508-070, SP, Brazil.
  • Sartorelli P; Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, SP, Brazil.
Pharmaceuticals (Basel) ; 15(10)2022 Sep 23.
Article en En | MEDLINE | ID: mdl-36297292
Gymnopilus consists of a widely distributed genus of basidiomycetes, especially in tropical regions of the world, such as Japan, Australia, Paraguay, and Brazil. This genus biosynthesizes interesting bioactive compounds, such as sesquiterpenoids, oligoisoprenoids, styrylpyrones, and lectins. In the present study, the aqueous extract of the basidiomata of Gymnopilus imperialis (Basidiomycota, Agaricomycetes, Agaricales, Hymenogastraceae) was obtained by using the accelerated solvent extraction (ASE) technique, followed by the precipitation of polysaccharide fraction with ethanol. Further purification by freeze-thawing processes, Fehling solution precipitation, and membrane dialysis with different pore sizes yield three main polysaccharide fractions (Gi-MRSW, Gi-PFME, and Gi-SFME). According to monosaccharide composition and 13C-NMR data, the Gi-MRSW and Gi-SFME fractions showed to be composed mainly of ß-glucans and Gi-PFME by a heterogalactan. Moreover, the immunomodulatory potential of Gi-MRSW was evaluated using RAW 264.7 murine macrophage as a study model. The nitric oxide production was significantly increased in treated samples, and the expression of inducible nitric oxide synthase (iNOS) showed that the fraction Gi-MRSW from G. imperialis induces the M1 polarization phenotype.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza