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MALDI-TOF Mass Spectrometry Online Identification of Trichophyton indotineae Using the MSI-2 Application.
Normand, Anne-Cécile; Moreno-Sabater, Alicia; Jabet, Arnaud; Hamane, Samia; Cremer, Geneviève; Foulet, Françoise; Blaize, Marion; Dellière, Sarah; Bonnal, Christine; Imbert, Sébastien; Brun, Sophie; Packeu, Ann; Bretagne, Stéphane; Piarroux, Renaud.
Afiliación
  • Normand AC; Service de Parasitologie-Mycologie, Hôpital La Pitié-Salpêtrière, AP-HP, 75013 Paris, France.
  • Moreno-Sabater A; Service de Parasitologie-Mycologie, Hôpital Saint-Antoine, AP-HP, 75012 Paris, France.
  • Jabet A; Centre d'Immunologie et des Maladies Infectieuses, (CIMI-PARIS), Inserm U1135, Sorbonne Université, 75013 Paris, France.
  • Hamane S; Service de Parasitologie-Mycologie, Hôpital La Pitié-Salpêtrière, AP-HP, 75013 Paris, France.
  • Cremer G; Hôpital Saint Louis Laboratoire de Parasitologie-Mycologie, Assistance Publique des Hôpitaux de Paris, 75010 Paris, France.
  • Foulet F; Laboratoire Bioclinic, 75009 Paris, France.
  • Blaize M; Service de Parasitologie-Mycologie, Hôpitaux Universitaires Henri Mondor, AP-HP, 94000 Créteil, France.
  • Dellière S; UR Dynamic 7380, UPEC, EnvA, USC ANSES, Faculté de Santé, 94000 Créteil, France.
  • Bonnal C; Service de Parasitologie-Mycologie, Hôpital La Pitié-Salpêtrière, AP-HP, 75013 Paris, France.
  • Imbert S; Sorbonne Université, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, 75013 Paris, France.
  • Brun S; Hôpital Saint Louis Laboratoire de Parasitologie-Mycologie, Assistance Publique des Hôpitaux de Paris, 75010 Paris, France.
  • Packeu A; Service de Parasitologie-Mycologie, Hôpital Bichat-Claude Bernard, AP-HP, 75018 Paris, France.
  • Bretagne S; Univ Bordeaux, Centre de Recherche Cardio-Thoracique de Bordeaux, Inserm UMR 1045, 33600 Bordeaux, France.
  • Piarroux R; CHU Bordeaux, Département de Parasitologie-Mycologie, CIC 1401, 33000 Bordeaux, France.
J Fungi (Basel) ; 8(10)2022 Oct 19.
Article en En | MEDLINE | ID: mdl-36294668
Trichophyton indotineae is an emerging pathogen which recently spread from India to Europe and that is more prone than other species of the Trichophyton mentagrophytes complex to show resistance to terbinafine, resulting in the necessity of rapid identification. Here, we improved the online MSI-2 MALDI-TOF identification tool in order to identify T. indotineae. By multiplying the culture conditions (2 culture media and 6 stages of growth) prior to protein extractions for both test isolates and reference strains, we added 142 references corresponding to 12 strains inside the T. mentagrophytes complex in the online MSI-2 database, of which 3 are T. indotineae strains. The resulting database was tested with 1566 spectra of 67 isolates from the T. mentagrophytes complex, including 16 T. indotineae isolates. Using the newly improved MSI-2 database, we increased the identification rate of T. indotineae from 5% to 96%, with a sensitivity of 99.6%. We also identified specific peaks (6834/6845 daltons and 10,634/10,680 daltons) allowing for the distinction of T. indotineae from the other species of the complex. Our improved version of the MSI-2 application allows for the identification of T. indotineae. This will improve the epidemiological knowledge of the spread of this species throughout the world and will help to improve patient care.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Fungi (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Fungi (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza