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Response of the Urothelial Carcinoma Cell Lines to Cisplatin.
Holícková, Andrea; Roska, Jan; Órásová, Eveline; Bruderová, Vladimíra; Palacka, Patrik; Jurkovicová, Dana; Chovanec, Miroslav.
Afiliación
  • Holícková A; Biomedical Research Center, Department of Genetics, Cancer Research Institute, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
  • Roska J; Biomedical Research Center, Department of Genetics, Cancer Research Institute, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
  • Órásová E; Biomedical Research Center, Department of Genetics, Cancer Research Institute, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
  • Bruderová V; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská Dolina, Ilkovicova 6, 842 15 Bratislava, Slovakia.
  • Palacka P; Biomedical Research Center, Department of Genetics, Cancer Research Institute, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
  • Jurkovicová D; National Cancer Institute, Klenová 1, 833 10 Bratislava, Slovakia.
  • Chovanec M; 2nd Department of Oncology, Faculty of Medicine, Comenius University in Bratislava, Klenová 1, 833 10 Bratislava, Slovakia.
Int J Mol Sci ; 23(20)2022 Oct 18.
Article en En | MEDLINE | ID: mdl-36293346
Cisplatin (CDDP)-based chemotherapy is the standard of care in patients with muscle-invasive bladder cancer. However, in a large number of cases, the disease becomes resistant or does not respond to CDDP, and thus progresses and disseminates. In such cases, prognosis of patients is very poor. CDDP manifests its cytotoxic effects mainly through DNA damage induction. Hence, response to CDDP is mainly dependent on DNA damage repair and tolerance mechanisms. Herein, we have examined CDDP response in a panel of the urothelial carcinoma cell (UCC) lines. We characterized these cell lines with regard to viability after CDDP treatment, as well as kinetics of induction and repair of CDDP-induced DNA damage. We demonstrate that repair of CDDP-induced DNA lesions correlates, at least to some extent, with CDDP sensitivity. Furthermore, we monitored expression of the key genes involved in selected DNA repair and tolerance mechanisms, nucleotide excision repair, homologous recombination and translesion DNA synthesis, and show that it differs in the UCC lines and positively correlates with CDDP resistance. Our data indicate that CDDP response in the UCC lines is dependent on DNA damage repair and tolerance factors, which may, therefore, represent valuable therapeutic targets in this malignancy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Carcinoma de Células Transicionales / Antineoplásicos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Carcinoma de Células Transicionales / Antineoplásicos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Suiza