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Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily.
Loth, Karine; Parisot, Nicolas; Paquet, Françoise; Terrasson, Hugo; Sivignon, Catherine; Rahioui, Isabelle; Ribeiro Lopes, Mélanie; Gaget, Karen; Duport, Gabrielle; Delmas, Agnès F; Aucagne, Vincent; Heddi, Abdelaziz; Calevro, Federica; da Silva, Pedro.
Afiliación
  • Loth K; Centre de Biophysique Moléculaire, CNRS UPR 4301, 45071 Orléans, France.
  • Parisot N; UFR Sciences et Techniques, Université d'Orléans, 45071 Orléans, France.
  • Paquet F; Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, 69621 Villeurbanne, France.
  • Terrasson H; Centre de Biophysique Moléculaire, CNRS UPR 4301, 45071 Orléans, France.
  • Sivignon C; Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, 69621 Villeurbanne, France.
  • Rahioui I; Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, 69621 Villeurbanne, France.
  • Ribeiro Lopes M; Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, 69621 Villeurbanne, France.
  • Gaget K; Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, 69621 Villeurbanne, France.
  • Duport G; Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, 69621 Villeurbanne, France.
  • Delmas AF; Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, 69621 Villeurbanne, France.
  • Aucagne V; Centre de Biophysique Moléculaire, CNRS UPR 4301, 45071 Orléans, France.
  • Heddi A; Centre de Biophysique Moléculaire, CNRS UPR 4301, 45071 Orléans, France.
  • Calevro F; Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, 69621 Villeurbanne, France.
  • da Silva P; Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, 69621 Villeurbanne, France.
Int J Mol Sci ; 23(20)2022 Oct 18.
Article en En | MEDLINE | ID: mdl-36293341
Aphids (Hemiptera: Aphidoidea) are among the most detrimental insects for agricultural plants, and their management is a great challenge in agronomical research. A new class of proteins, called Bacteriocyte-specific Cysteine-Rich (BCR) peptides, provides an alternative to chemical insecticides for pest control. BCRs were initially identified in the pea aphid Acyrthosiphon pisum. They are small disulfide bond-rich proteins expressed exclusively in aphid bacteriocytes, the insect cells that host intracellular symbiotic bacteria. Here, we show that one of the A. pisum BCRs, BCR4, displays prominent insecticidal activity against the pea aphid, impairing insect survival and nymphal growth, providing evidence for its potential use as a new biopesticide. Our comparative genomics and phylogenetic analyses indicate that BCRs are restricted to the aphid lineage. The 3D structure of BCR4 reveals that this peptide belongs to an as-yet-unknown structural class of peptides and defines a new superfamily of defensins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Áfidos / Insecticidas Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Áfidos / Insecticidas Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza