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Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor.
Wang, Wanxue; Pei, Cheng; Isachenko, Evgenia; Zhou, Yang; Wang, Mengying; Rahimi, Gohar; Liu, Wensheng; Mallmann, Peter; Isachenko, Vladimir.
Afiliación
  • Wang W; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
  • Pei C; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
  • Isachenko E; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
  • Zhou Y; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
  • Wang M; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
  • Rahimi G; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
  • Liu W; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
  • Mallmann P; NHC Key Laboratory of Male Reproduction and Genetics, Guangdong Provincial Reproductive Science Institute, Guangdong Provincial Fertility Hospital, Guangzhou 510062, China.
  • Isachenko V; Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931 Cologne, Germany.
Int J Mol Sci ; 23(20)2022 Oct 17.
Article en En | MEDLINE | ID: mdl-36293273
INTRODUCTION: The in vitro culture of primordial follicles is the only available option for preserving fertility in prepubertal girls with malignant tumors. The cultivation of primordial follicles in scaffolds as artificial ovaries is a promising approach for this. METHODS: Dissociated follicles were placed into an artificial ovarian scaffold composed of fibrinogen and thrombin. The follicles were cultured in a dish dedicated to live cell imaging and observed for growth using immunofluorescence and development via optical microscopy. The morphology of the follicles in the scaffold was three-dimensionally reconstructed using the Imaris software. Growth and development were also quantified. RESULTS: The morphology of artificial ovaries began to degrade over time. Within approximately 7 days, primordial follicles were activated and grew into secondary follicles. A comparison of optical and confocal microscopy results revealed the superior detection of live cells using confocal microscopy. The three-dimensional reconstruction of the confocal microscopy data enabled the automatic enumeration and evaluation of the overall morphology of many follicles. CONCLUSIONS: The novel artificial ovary-enabled primordial follicles to enter the growth cycle after activation and grow into secondary follicles. The use of a fibrin scaffold as a carrier preserves the developmental potential of primordial germ cells and is a potentially effective method for preserving fertility in prepubertal girls.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de la Fertilidad / Neoplasias Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de la Fertilidad / Neoplasias Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza