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Cobalt-mediated oxidative DNA damage and its prevention by polyphenol antioxidants.
Angelé-Martínez, Carlos; Murray, Joseph; Stewart, Paul A; Haines, Jennifer; Gaertner, Andrea A E; Brumaghim, Julia L.
Afiliación
  • Angelé-Martínez C; Department of Chemistry, Clemson University, Clemson, SC 29634-0973, USA.
  • Murray J; Department of Chemistry, Clemson University, Clemson, SC 29634-0973, USA.
  • Stewart PA; Department of Chemistry, Clemson University, Clemson, SC 29634-0973, USA.
  • Haines J; Department of Chemistry, Clemson University, Clemson, SC 29634-0973, USA.
  • Gaertner AAE; Department of Chemistry, Clemson University, Clemson, SC 29634-0973, USA.
  • Brumaghim JL; Department of Chemistry, Clemson University, Clemson, SC 29634-0973, USA. Electronic address: brumagh@clemson.edu.
J Inorg Biochem ; 238: 112024, 2023 01.
Article en En | MEDLINE | ID: mdl-36272187
Although cobalt is a required nutrient, it is toxic due to its ability to generate reactive oxygen species (ROS) and damage DNA. ROS generation by Co2+ often has been compared to that of Fe2+ or Cu+, disregarding the reduction potential differences among these metal ions. In plasmid DNA damage studies, a maximum of 15% DNA damage is observed with Co2+/H2O2 treatment (up to 50 µM and 400 µM, respectively) significantly lower than the 90% damage observed for Fe2+/H2O2 or Cu+/H2O2 treatment. However, when ascorbate is added to the Co2+/H2O2 system, a synergistic effect results in 90% DNA damage. DNA damage by Fe2+/H2O2 can be prevented by polyphenol antioxidants, but polyphenols both prevent and promote DNA damage by Cu+/H2O2. When tested for cobalt-mediated DNA damage affects, eight of ten polyphenols (epicatechin gallate, epigallocatechin gallate, propyl gallate, gallic acid, methyl-3,4,5-trihydroxybenzoate, methyl-4,5-dihydroxybenzoate, protocatechuic acid, and epicatechin) prevent cobalt-mediated DNA damage with IC50 values of 1.3 to 27 µM and two (epigallocatechin and vanillic acid) prevent little to no DNA damage. EPR studies demonstrate cobalt-mediated formation of •OH, O2•-, and •OOH, but not 1O2 in the presence of H2O2 and ascorbate. Epigallocatechin gallate and methyl-4,5-dihydroxybenzoate significantly reduce ROS generated by Co2+/H2O2/ascorbate, consistent with their prevention of cobalt-mediated DNA damage. Thus, while cobalt, iron, and copper are all d-block metal ions, cobalt ROS generation and its prevention is significantly different from that of iron and copper.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polifenoles / Antioxidantes Idioma: En Revista: J Inorg Biochem Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polifenoles / Antioxidantes Idioma: En Revista: J Inorg Biochem Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos