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Isolation and characterization of bacteriophage Ib_pec2 against shigatoxigenic Escherichia coli.
Inbaraj, Sophia; Angappan, M; Thomas, Prasad; Kumar, Manish; Irungbam, Karuna; Verma, Med Ram; Viswas, K Nagaleekar; Rawat, Mayank; Chaudhuri, Pallab.
Afiliación
  • Inbaraj S; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Angappan M; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Thomas P; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Kumar M; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Irungbam K; Animal Biochemistry Section, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Verma MR; Division of Livestock Economics, Statistics and Information Technology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Viswas KN; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Abhishek; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Rawat M; (Retd)Division of Biological Standardisation, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
  • Chaudhuri P; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
J Basic Microbiol ; 63(5): 472-480, 2023 May.
Article en En | MEDLINE | ID: mdl-36270976
This study was aimed to isolate and characterize bacteriophage against drug-resistant, shigatoxigenic Escherichia coli (STEC), one of the zoonotic, food-borne organisms associated with ruminants, mainly cattle. STEC were isolated (n = 35) from neonatal calves, dairy workers, and the surrounding environment and their antimicrobial resistance pattern was studied. Out of the 35 isolates tested, 17 isolates were found to be multidrug resistant to important antibiotics like ampicillin, amoxicillin-clavulanate, ciprofloxacin, streptomycin, and tetracycline. Bacteriophage namely Ib_pec2 was isolated against one of the STEC isolates and its morphology, genetic and proteomic characterization was done. Morphological analysis by TEM revealed bacteriophages belonging to myoviridae family. The genetic characterization of g23 gene revealed that the bacteriophage belonged to Tequatrovirus of myoviridae family. Proteomic analysis was able to identify five proteins identical to Tequatrovirus of myoviridae family. One-step growth curve experiment revealed a latency period of 40 min and a burst size of 893 pfu/bacteria. Temperature and pH ranging from 40°C to 50°C, pH 6-8, respectively. Phage could able to lyse majority of the STEC isolates. STEC are commensal organisms in the gastrointestinal tract of ruminants but are pathogenic in humans. Bacteriophages can be used as alternatives to antibiotics to control bacterial growth in ruminants and prevent its further spillage in the environment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals / Humans Idioma: En Revista: J Basic Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals / Humans Idioma: En Revista: J Basic Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania