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Sublethal cadmium exposure in the freshwater snail Lymnaea stagnalis meets a deficient, poorly responsive metallothionein system while evoking oxidative and cellular stress.
Gnatyshyna, Lesya; Khoma, Vira; Martinyuk, Viktoria; Matskiv, Tetyana; Pedrini-Martha, Veronika; Niederwanger, Michael; Stoliar, Oksana; Dallinger, Reinhard.
Afiliación
  • Gnatyshyna L; I.Ya. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine; Ternopil Volodymyr Hnatiuk National Pedagogical University, Ternopil, Ukraine. Electronic address: gnatyshynall@tdmu.edu.ua.
  • Khoma V; Ternopil Volodymyr Hnatiuk National Pedagogical University, Ternopil, Ukraine. Electronic address: khomav@tnpu.edu.ua.
  • Martinyuk V; Ternopil Volodymyr Hnatiuk National Pedagogical University, Ternopil, Ukraine.
  • Matskiv T; I.Ya. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine; Ternopil Volodymyr Hnatiuk National Pedagogical University, Ternopil, Ukraine. Electronic address: mackiw@tdmu.edu.ua.
  • Pedrini-Martha V; Institute of Zoology, University and Center of Molecular Biosciences, Innsbruck, Austria. Electronic address: Veronika.Pedrini-Martha@uibk.ac.at.
  • Niederwanger M; Institute of Zoology, University and Center of Molecular Biosciences, Innsbruck, Austria. Electronic address: Michael.Niederwanger@uibk.ac.at.
  • Stoliar O; Ternopil Volodymyr Hnatiuk National Pedagogical University, Ternopil, Ukraine. Electronic address: Oksana.Stolyar@tnpu.edu.ua.
  • Dallinger R; Institute of Zoology, University and Center of Molecular Biosciences, Innsbruck, Austria. Electronic address: Reinhard.dallinger@uibk.ac.at.
Comp Biochem Physiol C Toxicol Pharmacol ; 263: 109490, 2023 Jan.
Article en En | MEDLINE | ID: mdl-36265756
The Great Pond snail Lymnaea stagnalis (Gastropoda, Hygrophila) is a wide-spread freshwater gastropod, being considered as a model organism for research in many fields of biology, including ecotoxicology. The aim of the present study was to explore the Cd sensitivity of L. stagnalis through the measurement of a biomarker battery for oxidative, toxic and cellular stress. The interpretation of biomarker parameters occurred against the background of a truncated metallothionein protein with a limited Cd-binding capacity. Individuals of L. stagnalis were exposed through 14 days to uncontaminated water (controls) or to low (30 µg · L-1) or high (50 µg · L-1) Cd concentrations. The digestive gland of control and low-Cd exposed snails was processed for transcriptional analysis of the Metallothionein (MT) gene expression, and for determination of biomarkers for oxidative stress, toxicity and cellular stress. Digestive gland supernatants of high-Cd exposed snails were subjected to chromatography and subsequent analysis by spectrophotometry. It was shown that the MT system of L. stagnalis is functionally deficient, with a poor Cd responsiveness at both, the transcriptional and the protein expression levels. Instead, L. stagnalis appears to rely on alternative detoxification mechanisms such as Cd binding by phytochelatins and metal inactivation by compartmentalization within the lysosomal system. In spite of this, however, traces of Cd apparently leak out of the pre-determined detoxification pathways, leading to adverse effects, which is clearly indicated by biomarkers of oxidative and cellular stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lymnaea / Metalotioneína Límite: Animals / Humans Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lymnaea / Metalotioneína Límite: Animals / Humans Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos