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The genome and lifestage-specific transcriptomes of a plant-parasitic nematode and its host reveal susceptibility genes involved in trans-kingdom synthesis of vitamin B5.
Siddique, Shahid; Radakovic, Zoran S; Hiltl, Clarissa; Pellegrin, Clement; Baum, Thomas J; Beasley, Helen; Bent, Andrew F; Chitambo, Oliver; Chopra, Divykriti; Danchin, Etienne G J; Grenier, Eric; Habash, Samer S; Hasan, M Shamim; Helder, Johannes; Hewezi, Tarek; Holbein, Julia; Holterman, Martijn; Janakowski, Slawomir; Koutsovoulos, Georgios D; Kranse, Olaf P; Lozano-Torres, Jose L; Maier, Tom R; Masonbrink, Rick E; Mendy, Badou; Riemer, Esther; Sobczak, Miroslaw; Sonawala, Unnati; Sterken, Mark G; Thorpe, Peter; van Steenbrugge, Joris J M; Zahid, Nageena; Grundler, Florian; Eves-van den Akker, Sebastian.
Afiliación
  • Siddique S; Department of Entomology and Nematology, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA.
  • Radakovic ZS; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Hiltl C; P.H. Petersen Saatzucht Lundsgaard GmbH, D-24977, Grundhof, Germany.
  • Pellegrin C; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Baum TJ; The Crop Science Centre, Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, UK.
  • Beasley H; Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA.
  • Bent AF; The Crop Science Centre, Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, UK.
  • Chitambo O; Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, 53706, USA.
  • Chopra D; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Danchin EGJ; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Grenier E; Université Côte d'Azur, INRAE, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France.
  • Habash SS; IGEPP, INRAE, Institut Agro, Université Rennes, 35650, Le Rheu, France.
  • Hasan MS; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Helder J; BASF Vegetable Seeds, Napoleonsweg 152, 6083, AB, Nunhem, The Netherlands.
  • Hewezi T; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Holbein J; Laboratory of Nematology, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.
  • Holterman M; Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA.
  • Janakowski S; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Koutsovoulos GD; Laboratory of Nematology, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.
  • Kranse OP; Solynta, Dreijenlaan 2, 6703, HA, Wageningen, The Netherlands.
  • Lozano-Torres JL; Department of Botany, Institute of Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-787, Warsaw, Poland.
  • Maier TR; Université Côte d'Azur, INRAE, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France.
  • Masonbrink RE; The Crop Science Centre, Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, UK.
  • Mendy B; Laboratory of Nematology, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.
  • Riemer E; Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA.
  • Sobczak M; Genome Informatics Facility, Iowa State University, Ames, IA, 50010, USA.
  • Sonawala U; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Sterken MG; Rheinische Friedrich-Wilhelms-University of Bonn, INRES - Molecular Phytomedicine, Karlrobert- Kreiten-Straße 13, D-53115, Bonn, Germany.
  • Thorpe P; Department of Botany, Institute of Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-787, Warsaw, Poland.
  • van Steenbrugge JJM; The Crop Science Centre, Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, UK.
  • Zahid N; Laboratory of Nematology, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.
  • Grundler F; Mackenzie Institute for Early Diagnosis, School of Medicine, University of St Andrews, North Haugh, St Andrews, KY16 9TF, UK.
  • Eves-van den Akker S; Laboratory of Nematology, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.
Nat Commun ; 13(1): 6190, 2022 10 19.
Article en En | MEDLINE | ID: mdl-36261416
Plant-parasitic nematodes are a major threat to crop production in all agricultural systems. The scarcity of classical resistance genes highlights a pressing need to find new ways to develop nematode-resistant germplasm. Here, we sequence and assemble a high-quality phased genome of the model cyst nematode Heterodera schachtii to provide a platform for the first system-wide dual analysis of host and parasite gene expression over time, covering all major parasitism stages. Analysis of the hologenome of the plant-nematode infection site identified metabolic pathways that were incomplete in the parasite but complemented by the host. Using a combination of bioinformatic, genetic, and biochemical approaches, we show that a highly atypical completion of vitamin B5 biosynthesis by the parasitic animal, putatively enabled by a horizontal gene transfer from a bacterium, is required for full pathogenicity. Knockout of either plant-encoded or now nematode-encoded steps in the pathway significantly reduces parasitic success. Our experiments establish a reference for cyst nematodes, further our understanding of the evolution of plant-parasitism by nematodes, and show that congruent differential expression of metabolic pathways in the infection hologenome represents a new way to find nematode susceptibility genes. The approach identifies genome-editing-amenable targets for future development of nematode-resistant crops.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parásitos / Tylenchida / Quistes Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parásitos / Tylenchida / Quistes Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido