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An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma.
Sparvoli, Daniela; Delabre, Jason; Penarete-Vargas, Diana Marcela; Kumar Mageswaran, Shrawan; Tsypin, Lev M; Heckendorn, Justine; Theveny, Liam; Maynadier, Marjorie; Mendonça Cova, Marta; Berry-Sterkers, Laurence; Guérin, Amandine; Dubremetz, Jean-François; Urbach, Serge; Striepen, Boris; Turkewitz, Aaron P; Chang, Yi-Wei; Lebrun, Maryse.
Afiliación
  • Sparvoli D; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Delabre J; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Penarete-Vargas DM; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Kumar Mageswaran S; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Tsypin LM; Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.
  • Heckendorn J; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Theveny L; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Maynadier M; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Mendonça Cova M; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Berry-Sterkers L; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Guérin A; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Dubremetz JF; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
  • Urbach S; IGF, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Striepen B; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Turkewitz AP; Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.
  • Chang YW; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Lebrun M; Laboratory of Pathogen Host Interactions, UMR 5235 CNRS, Université de Montpellier, Montpellier, France.
EMBO J ; 41(22): e111158, 2022 11 17.
Article en En | MEDLINE | ID: mdl-36245278
Apicomplexan parasites possess secretory organelles called rhoptries that undergo regulated exocytosis upon contact with the host. This process is essential for the parasitic lifestyle of these pathogens and relies on an exocytic machinery sharing structural features and molecular components with free-living ciliates. However, how the parasites coordinate exocytosis with host interaction is unknown. Here, we performed a Tetrahymena-based transcriptomic screen to uncover novel exocytic factors in Ciliata and conserved in Apicomplexa. We identified membrane-bound proteins, named CRMPs, forming part of a large complex essential for rhoptry secretion and invasion in Toxoplasma. Using cutting-edge imaging tools, including expansion microscopy and cryo-electron tomography, we show that, unlike previously described rhoptry exocytic factors, TgCRMPs are not required for the assembly of the rhoptry secretion machinery and only transiently associate with the exocytic site-prior to the invasion. CRMPs and their partners contain putative host cell-binding domains, and CRMPa shares similarities with GPCR proteins. Collectively our data imply that the CRMP complex acts as a host-molecular sensor to ensure that rhoptry exocytosis occurs when the parasite contacts the host cell.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma Tipo de estudio: Prognostic_studies Idioma: En Revista: EMBO J Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma Tipo de estudio: Prognostic_studies Idioma: En Revista: EMBO J Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido