Rapid liquid chromatographic determination of dimetridazole and ipronidazole in swine feed.
J Assoc Off Anal Chem
; 70(4): 626-30, 1987.
Article
en En
| MEDLINE
| ID: mdl-3624165
A simple and rapid method is described for the determination of dimetridazole (DMZ) and ipronidazole (IPR) in swine feeds at various levels (0.11-110 ppm). The drugs are released from feed by prewetting with a buffer, followed by extraction with either methanol or methylene chloride, depending on the drug level; if necessary, an acid-base cleanup is used before the liquid chromatographic analysis. The analytes are separated on a C18 column and monitored at 320 nm for detection and quantitation. Recoveries of DMZ from several feed formulations averaged 108% at the 92.8 ppm level with a standard deviation (SD) of 4.00% and a coefficient of variation (CV) of 3.70%, 101% at the 11.2 ppm level with an SD of 11.9% and a CV of 11.8%, and 100% at the 0.112 ppm level with an SD of 9.27% and a CV of 9.25%. Recoveries of IPR averaged 77.1% at the 12.9 ppm level with an SD of 1.75% and a CV of 2.27%; IPR recoveries averaged 35.2% at the 0.129 ppm level with an SD of 3.39% and a CV of 9.63%.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dimetridazol
/
Ipronidazol
/
Alimentación Animal
/
Nitroimidazoles
Límite:
Animals
Idioma:
En
Revista:
J Assoc Off Anal Chem
Año:
1987
Tipo del documento:
Article
Pais de publicación:
Estados Unidos