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Sulfhemoglobin under the spotlight - Detection and characterization of SHb and HbFeIII-SH.
Stepanenko, Tetiana; Zajac, Grzegorz; Czajkowski, Artur; Rutkowska, Wiktoria; Górecki, Andrzej; Marzec, Katarzyna Maria; Dybas, Jakub.
Afiliación
  • Stepanenko T; Jagiellonian University, Faculty of Chemistry, 2 Gronostajowa Str., 30-387 Krakow, Poland; Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), 14 Bobrzynskiego Str., 30-348 Krakow, Poland.
  • Zajac G; Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), 14 Bobrzynskiego Str., 30-348 Krakow, Poland.
  • Czajkowski A; Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology (BBB), 7 Gronostajowa Str., 30-387 Krakow, Poland.
  • Rutkowska W; Jagiellonian University, Faculty of Chemistry, 2 Gronostajowa Str., 30-387 Krakow, Poland.
  • Górecki A; Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology (BBB), 7 Gronostajowa Str., 30-387 Krakow, Poland.
  • Marzec KM; Lukasiewicz Research Network - Krakow Institute of Technology, 73 Zakopianska St., 30-418 Krakow, Poland.
  • Dybas J; Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), 14 Bobrzynskiego Str., 30-348 Krakow, Poland. Electronic address: jakub.dybas@uj.edu.pl.
Biochim Biophys Acta Mol Cell Res ; 1870(1): 119378, 2023 01.
Article en En | MEDLINE | ID: mdl-36220452
Sulfhemoglobinemia is an incurable disease caused by an overdose of sulfur-containing drugs with oxidizing properties. Its diagnosis remains hindered due to the similarity of symptoms to other pathological state - methemoglobinemia, as well as contradictory information on the structure and characteristics of sulfhemoglobin. Herein, we present sulfhemoglobinemia model on living functional human erythrocytes, designed to recreate processes which could take place in a patient body in order to complement missing information and highlight distinctiveness of two hemoglobin (Hb) adducts formed after interaction with sulfur donors. Employed techniques, UV-Vis absorption, Raman, Fourier transformed infrared (FT-IR) and electronic circular dichroism (ECD) spectroscopies, allowed to distinguish and characterize Hb adduct with sulfur atom bounded directly to the iron ion (HbFeIII-SH), and irreversibly connected to the porphyrin ring (SHb - sulfhemoglobin). Presented herein results provided also new evidence on formation of both these hemoglobin adducts inside functional erythrocytes under oxidative conditions and during sulfur-containing drug presence, what can be further translated into future physiological studies. Moreover, we found that sulfur attachment to the porphyrin ring altered Hb structure and lead to changes in protein packing inside RBCs, eventually. Interestingly, measurement of blood drop smear by Raman spectroscopy occurred the most accurate method to differentiate HbFeIII-SH and SHb, indicating potential of this technique in sulfhemoglobinemia diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Porfirinas / Sulfohemoglobinemia Límite: Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Res Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Porfirinas / Sulfohemoglobinemia Límite: Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Res Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos