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Brushite nanoparticles based electrochemical sensor for detection of uric acid, xanthine, hypoxanthine and caffeine.
Sudhan, N; Anitta, S; Meenakshi, S; Sekar, C.
Afiliación
  • Sudhan N; Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, 630 004, Tamilnadu, India; Department of Chemistry, Thiagarajar College, Madurai, 625009, Tamilnadu, India.
  • Anitta S; Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, 630 004, Tamilnadu, India.
  • Meenakshi S; Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, 630 004, Tamilnadu, India.
  • Sekar C; Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, 630 004, Tamilnadu, India. Electronic address: Sekar2025@alagappauniversity.ac.in.
Anal Biochem ; 659: 114947, 2022 12 15.
Article en En | MEDLINE | ID: mdl-36216144
The research in the field of biosensors has recently been focused on the design and development of functional electrode materials that can respond to changes in their biochemical environment. Here, we report the synthesis of dicalcium phosphate dihydrate (DCPD), also known as brushite (CaHPO4·2H2O) by soft chemical method and its application for electrochemical sensing of four different analytes. Phase purity, structure, chemical composition and surface morphology of the synthesized nanoparticles have been investigated using powder XRD, FTIR, SEM, XPS and HRTEM methods. Electrochemical sensor was prepared by modifying GCE with brushite and the modified electrodes were successfully used for either independent or simultaneous determination of uric acid, xanthine, hypoxanthine and caffeine in their mixture. The brushite/GCE exhibited four strong well-defined separate peaks corresponding to the oxidation of UA, XN, HXN and CF in phosphate buffer saline (PBS) at pH 7.4. The fabricated electrode showed low detection limits (S/N = 3) of 0.576, 1.0, 0.076 and 1.26 µM for UA, XN, HXN and CF respectively. Practical application of the fabricated electrode has been demonstrated by determining UA, XN, HXN and CF in human urine and coffee samples by direct method. The brushite offers scope for fabrication of sensor systems for implantable medical applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Úrico / Nanopartículas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Biochem Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Úrico / Nanopartículas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Biochem Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos