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A bone-on-a-chip collagen hydrogel-based model using pre-differentiated adipose-derived stem cells for personalized bone tissue engineering.
Alamán-Díez, Pilar; García-Gareta, Elena; Arruebo, Manuel; Pérez, María Ángeles.
Afiliación
  • Alamán-Díez P; Multiscale in Mechanical and Biological Engineering, Aragón Institute of Engineering Research (I3A), Aragón Institute of Healthcare Research (IIS Aragón), University of Zaragoza, Zaragoza, Spain.
  • García-Gareta E; Multiscale in Mechanical and Biological Engineering, Aragón Institute of Engineering Research (I3A), Aragón Institute of Healthcare Research (IIS Aragón), University of Zaragoza, Zaragoza, Spain.
  • Arruebo M; Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, UK.
  • Pérez MÁ; Aragón Institute of Nanoscience and Materials (INMA), Consejo Superior de Investigaciones Científicas (CSIC), University of Zaragoza, Zaragoza, Spain.
J Biomed Mater Res A ; 111(1): 88-105, 2023 01.
Article en En | MEDLINE | ID: mdl-36205241
Mesenchymal stem cells have contributed to the continuous progress of tissue engineering and regenerative medicine. Adipose-derived stem cells (ADSC) possess many advantages compared to other origins including easy tissue harvesting, self-renewal potential, and fast population doubling time. As multipotent cells, they can differentiate into osteoblastic cell linages. In vitro bone models are needed to carry out an initial safety assessment in the study of novel bone regeneration therapies. We hypothesized that 3D bone-on-a-chip models containing ADSC could closely recreate the physiological bone microenvironment and promote differentiation. They represent an intermedium step between traditional 2D-in vitro and in vivo experiments facilitating the screening of therapeutic molecules while saving resources. Herein, we have differentiated ADSC for 7 and 14 days and used them to fabricate in vitro bone models by embedding the pre-differentiated cells in a 3D collagen matrix placed in a microfluidic chip. Osteogenic markers such as alkaline phosphatase activity, calcium mineralization, changes on cell morphology, and expression of specific proteins (bone sialoprotein 2, dentin matrix acidic phosphoprotein-1, and osteocalcin) were evaluated to determine cell differentiation potential and evolution. This is the first miniaturized 3D-in vitro bone model created from pre-differentiated ADSC embedded in a hydrogel collagen matrix which could be used for personalized bone tissue engineering.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrogeles / Ingeniería de Tejidos Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrogeles / Ingeniería de Tejidos Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos