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Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement.
Melo, Leyva Cecília Vieira de; Souza, Felipe Corrêa Rezende de; Baccin, Amanda de Oliveira; Mota, Dan Jessé Gonçalves da; Pereira-Chioccola, Vera Lucia; Pinto, Pedro Luiz Silva.
Afiliación
  • Melo LCV; Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, Núcleo de Enteroparasitas, São Paulo, SP, Brasil.
  • Souza FCR; Secretaria Estadual da Saúde de São Paulo, Coordenadoria do Controle de Doenças, Programa de Pós-Graduação, São Paulo, SP, Brasil.
  • Baccin AO; Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, Núcleo de Enteroparasitas, São Paulo, SP, Brasil.
  • Mota DJGD; Secretaria Estadual da Saúde de São Paulo, Coordenadoria do Controle de Doenças, Programa de Pós-Graduação, São Paulo, SP, Brasil.
  • Pereira-Chioccola VL; Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, Núcleo de Enteroparasitas, São Paulo, SP, Brasil.
  • Pinto PLS; Secretaria Estadual da Saúde de São Paulo, Coordenadoria do Controle de Doenças, Programa de Pós-Graduação, São Paulo, SP, Brasil.
Mem Inst Oswaldo Cruz ; 117: e220086, 2022.
Article en En | MEDLINE | ID: mdl-36197401
BACKGROUND: Angiostrongylus cantonensis is the etiological agent of neuroangiostrongyliasis in humans, which is developed in gastropods and vertebrate species, mainly rodents. Human transmission occurs through consumption of molluscs and paratenic hosts infected with L3, and the migration of larvae to the central nervous system causes eosinophilic meningitis. Laboratory diagnosis is based on molecular and immunological tests, using young or adult females as a source of antigens. However, these tests give positive results only after several weeks of symptoms onset and also cross-reactions with others parasite infections may occur. OBJECTIVES: The purpose of this work was to study different antigenic preparations of distinct evolutionary phases of A. cantonensis, in order to improve serological techniques for disease immunodiagnosis. METHODS: For this purpose, antigenic fractions of different evolutionary forms were evaluated by Dot-enzyme-linked immunosorbent assay (Dot-ELISA) and Western blot using serum samples. FINDINGS: All analysed fractions showed reactivity with serum samples from patients with neuroangiostrongyliasis, especially female membrane alkaline (FAM) and female soluble alkaline (FAS) fractions together with female soluble saline (FSS), improving the technique specificity. MAIN CONCLUSIONS: The results point to the possibility of use of raw female antigens in association with alkaline membrane antigens extracted from adult worms to aid in diagnosis and helps initiate neuroangiostrongyliasis surveillance and control actions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Strongylida / Angiostrongylus cantonensis / Meningitis Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Mem Inst Oswaldo Cruz Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Strongylida / Angiostrongylus cantonensis / Meningitis Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Mem Inst Oswaldo Cruz Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil