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Oat Seedlings Extract Inhibits RANKL-Induced c-Fos/NFATc1 Transcription Factors in the Early Stage of Osteoclast Differentiation.
Yang, Ji Yeong; Kim, Shin-Hye; Lee, HanGyeol; Lee, Kwang-Sik; Song, Seung-Yeob; Lee, Mi Ja; Kim, Hyun Young; Lim, So-An; Park, Kie-In; Choi, Sik-Won; Seo, Woo Duck.
Afiliación
  • Yang JY; Crop Foundation Research Division, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Republic of Korea.
  • Kim SH; Forest Biomaterials Research Center, National Institute of Forest Science (NIFoS), Korea Forest Service (KFS), Jinju 52817, Republic of Korea.
  • Lee H; Department of Biological Sciences, College of Natural Science, Jeonbuk National University, Jeonju 54896, Republic of Korea.
  • Lee KS; Crop Foundation Research Division, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Republic of Korea.
  • Song SY; Department of Biological Sciences, College of Natural Science, Jeonbuk National University, Jeonju 54896, Republic of Korea.
  • Lee MJ; Fruit Research Division, National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Wanju 55365, Republic of Korea.
  • Kim HY; Crop Foundation Research Division, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Republic of Korea.
  • Lim SA; Crop Foundation Research Division, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Republic of Korea.
  • Park KI; Crop Foundation Research Division, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Republic of Korea.
  • Choi SW; Forest Biomaterials Research Center, National Institute of Forest Science (NIFoS), Korea Forest Service (KFS), Jinju 52817, Republic of Korea.
  • Seo WD; Department of Forest Environmental Resources, College of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
Evid Based Complement Alternat Med ; 2022: 5372459, 2022.
Article en En | MEDLINE | ID: mdl-36193131
Osteoporosis is a common disease that increases the risk of fractures due to decreased bone density and weakens the bone microstructure. Preventing and diagnosing osteoporosis using the available drugs can be a costly affair with possible side effects. Therefore, natural product-derived therapeutics are promising alternatives. Our study demonstrated that the oat seedlings' extract (OSE) inhibited the receptor activator of the nuclear factor κB ligand (RANKL)-induced osteoclastogenesis from the bone marrow-derived macrophages (BMMs). The OSE treatment significantly attenuated the RANKL-mediated induction of the tartrate-resistant acid phosphatase (TRAP) activity as well as the number of TRAP-positive (TRAP+) multinucleated cells (MNCs) counted through the TRAP staining in a dose-dependent manner. It was also confirmed that the OSE suppressed the formation of the TRAP + MNCs in the early stage of differentiation and not in the middle and late stages. The results of the real-time quantitative polymerase chain reaction (qPCR) and the western blotting showed that the OSE dramatically inhibited the mRNA and protein expressions of the osteoclastogenesis-mediated transcription factors such as the c-Fos and the nuclear factor-activated T cells c1 (NFATc1). In addition, the OSE strongly attenuated the mRNA induction of the c-Fos/NFATc1-dependent molecules such as the TRAP, the osteoclast-associatedimmunoglobulin-like receptor (OSCAR), the dendritic cell-specific transmembrane protein (DC-STAMP), and the cathepsin K. These results suggest that the naturally derived OSE may be useful for preventing bone diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos