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Quantitative analysis of RT-PCR test results for SARS-CoV-2 diagnostics across Poland during COVID-19 pandemic: Comparison between early stage and major pandemic waves in 2020 and 2021 with reference to SARS-CoV-2 variants.
Gierczynski, Rafal; Czerw, Aleksandra; Juszczyk, Grzegorz; Charkiewicz, Radoslaw; Niklinski, Jacek; Majewski, Piotr; Reszec, Joanna; Piatyszek, Piotr; Baniecki, Hubert; Biecek, Przemyslaw; Henry, Brandon Michael.
Afiliación
  • Gierczynski R; Department of Bacteriology and Biocontamination Control, National Institute of Public Health, National Institute of Hygiene, Warsaw, Poland.
  • Czerw A; Department of Economic and System Analyses, National Institute of Public Health, National Institute of Hygiene, Warsaw, Poland; Department of Health Economics and Medical Law, Medical University of Warsaw, Warsaw, Poland.
  • Juszczyk G; Department of Public Health, Medical University of Warsaw, Warsaw, Poland.
  • Charkiewicz R; Center of Experimental Medicine, Medical University of Bialystok, Bialystok, Poland.
  • Niklinski J; Department of Clinical Molecular Biology, Medical University of Bialystok, Bialystok, Poland.
  • Majewski P; Department of Clinical Molecular Biology, Medical University of Bialystok, Bialystok, Poland.
  • Reszec J; Department of Medical Pathomorphology, Medical University of Bialystok, Bialystok, Poland.
  • Piatyszek P; Faculty of Mathematics and Information Sciences, Warsaw University of Technology, Warsaw, Poland.
  • Baniecki H; Faculty of Mathematics and Information Sciences, Warsaw University of Technology, Warsaw, Poland.
  • Biecek P; Faculty of Mathematics and Information Sciences, Warsaw University of Technology, Warsaw, Poland.
  • Henry BM; Clinical Laboratory, Division of Nephrology and Hypertension, Children's Hospital Medical Center, Cincinnati, OH, USA. Electronic address: brandon.henry@cchmc.org.
Adv Med Sci ; 67(2): 386-392, 2022 Sep.
Article en En | MEDLINE | ID: mdl-36191361
PURPOSE: From April to September 2020, Poland was minimally affected by COVID-19 compared to other EU countries. We aimed to investigate the risks of false reverse transcription polymerase chain reaction (RT-PCR) results during the first wave (compared to later waves), that rises when cycle threshold (Ct) of positive result is close to limit of detection (LOD). MATERIALS/METHODS: We analyzed Ct values of SARS-CoV-2 positive RT-PCR results of 7726 patients in Poland from April-September 2020. SARS-CoV-2 positive RT-PCR results of 14,534 patients in the 2nd-3rd wave and 10,861 patients in the 4th-5th pandemic waves were used. Statistical analysis was based on one-way analysis of variance. To verify, 95% confidence intervals with Bonferroni correction were computed. Incidence of SARS-CoV-2 variants in Poland was analyzed using Whole Genome Sequencing from 923 (3.6%) patients. RESULTS: The mean Ct of RT-PCR positive test results analyzed ranged between 22.89 and 26.71 depending on the month of the results collection. The differences between months were significant (p â€‹< â€‹0.001). Differences in Ct were observed between age groups, with younger patients displaying higher Ct values, however, major trends over time were paralleled between age groups. CONCLUSIONS: The mean Ct of the tested RT-PCR positive test results was lower than 35 which is considered an upper borderline for reliable positive results of the assay. Therefore, most COVID-19 cases recorded in Poland from April to September 2020 were detected with minor risks of inaccuracy. Data from a single center exhibited greater consistency for both virus Ct level and SARS-CoV-2 virus variant identification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Adv Med Sci Asunto de la revista: MEDICINA Año: 2022 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Adv Med Sci Asunto de la revista: MEDICINA Año: 2022 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos