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Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis.
Fan, Lusheng; Gao, Bin; Xu, Ye; Flynn, Nora; Le, Brandon; You, Chenjiang; Li, Shaofang; Achkar, Natalia; Manavella, Pablo A; Yang, Zhenbiao; Chen, Xuemei.
Afiliación
  • Fan L; FAFU-UCR Joint Center for Horticultural Biology & Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
  • Gao B; Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA; 92521.
  • Xu Y; Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA; 92521.
  • Flynn N; Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA; 92521.
  • Le B; Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA; 92521.
  • You C; Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA; 92521.
  • Li S; State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
  • Achkar N; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Manavella PA; Instituto de Agrobiotecnologia del Litoral (CONICET-UNL-FBCB), Santa Fe 3000, Argentina.
  • Yang Z; Instituto de Agrobiotecnologia del Litoral (CONICET-UNL-FBCB), Santa Fe 3000, Argentina.
  • Chen X; Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA; 92521.
Proc Natl Acad Sci U S A ; 119(41): e2208415119, 2022 10 11.
Article en En | MEDLINE | ID: mdl-36191209
MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP assembly factor in yeast and humans, not only acts in splicing but also promotes miRNA biogenesis. AAR2 interacts with the microprocessor component hyponastic leaves 1 (HYL1) in the cytoplasm, nucleus, and dicing bodies. In aar2 mutants, abundance of nonphosphorylated HYL1, the active form of HYL1, and the number of HYL1-labeled dicing bodies are reduced. Primary miRNA (pri-miRNA) accumulation is compromised despite normal promoter activities of MIR genes in aar2 mutants. RNA decay assays show that the aar2-1 mutation leads to faster degradation of pri-miRNAs in a HYL1-dependent manner, which reveals a previously unknown and negative role of HYL1 in miRNA biogenesis. Taken together, our findings reveal a dual role of AAR2 in miRNA biogenesis and pre-messenger RNA splicing.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis / MicroARNs Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis / MicroARNs Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos