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Optically traceable PLGA-silica nanoparticles for cell-triggered doxorubicin delivery.
Raj, Ritu; Pinto, Sandra N; Crucho, Carina I C; Das, Surajit; Baleizão, Carlos; Farinha, José Paulo S.
Afiliación
  • Raj R; Centro de Química Estrutural, Institute of Molecular Sciences, and Department of Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; Department of Life Science, Laboratory of Environmental Microbiology and Ecology (LEnME), National I
  • Pinto SN; iBB-Institute of Bioengineering and Biosciences, i4HB-Institute for Health and Bioeconomy, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: sandrapinto@tecnico.ulisboa.pt.
  • Crucho CIC; iBB-Institute of Bioengineering and Biosciences, i4HB-Institute for Health and Bioeconomy, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: carina.crucho@tecnico.ulisboa.pt.
  • Das S; Department of Life Science, Laboratory of Environmental Microbiology and Ecology (LEnME), National Institute of Technology Rourkela, Rourkela 769 008, Odisha, India. Electronic address: surajit@nitrkl.ac.in.
  • Baleizão C; Centro de Química Estrutural, Institute of Molecular Sciences, and Department of Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: carlos.baleizao@tecnico.ulisboa.pt.
  • Farinha JPS; Centro de Química Estrutural, Institute of Molecular Sciences, and Department of Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: farinha@tecnico.ulisboa.pt.
Colloids Surf B Biointerfaces ; 220: 112872, 2022 Dec.
Article en En | MEDLINE | ID: mdl-36179611
Fluorescent silica nanoparticles with a polymer shell of poly (D, L-lactide-co-glycolide) (PLGA) can provide traceable cell-triggered delivery of the anticancer drug doxorubicin (DOX), protecting the cargo while in transit and releasing it only intracellularly. PLGA with 50:50 lactide:glycolide ratio was grown by surface-initiated ring-opening polymerization (ROP) from silica nanoparticles of ca. 50 nm diameter, doped with a perylenediimide (PDI) fluorescent dye anchored to the silica structure. After loading DOX, release from the core-shell particles was evaluated in solution at physiological pH (7.4), and in human breast cancer cells (MCF-7) after internalization. The hybrid silica-PLGA nanoparticles can accommodate a large cargo of DOX, and the release in solution (PBS) due to PLGA hydrolysis is negligible for at least 72 h. However, once internalized in MCF-7 cells, the nanoparticles release the DOX cargo by degradation of the PLGA. Accumulation of DOX in the nucleus causes cell apoptosis, with the drug-loaded nanoparticles found to be as potent as free DOX. Our fluorescently traceable hybrid silica-PLGA nanoparticles with cell-triggered cargo release offer excellent prospects for the controlled delivery of anticancer drugs, protecting the cargo while in transit and efficiently releasing the drug once inside the cell.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas / Antineoplásicos Límite: Humans Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas / Antineoplásicos Límite: Humans Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos