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Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics.
Centurion, V B; Campanaro, S; Basile, A; Treu, L; Oliveira, V M.
Afiliación
  • Centurion VB; Microbial Resources Division, Research Center for Chemistry, Biology, and Agriculture (CPQBA), State University of Campinas - UNICAMP, Paulínia, SP CEP 13081-970, Brazil; Biology Institute, State University of Campinas - UNICAMP, Campinas, SP CEP 13083-862, Brazil. Electronic address: vborincenturio
  • Campanaro S; Department of Biology, University of Padova, Via U. Bassi 58/b, 35121 Padua, Italy; CRIBI Biotechnology Center, University of Padova, 35131 Padua, Italy. Electronic address: stefano.campanaro@unipd.it.
  • Basile A; Department of Biology, University of Padova, Via U. Bassi 58/b, 35121 Padua, Italy. Electronic address: arianna.basile@phd.unipd.it.
  • Treu L; Department of Biology, University of Padova, Via U. Bassi 58/b, 35121 Padua, Italy. Electronic address: laura.treu@unipd.it.
  • Oliveira VM; Microbial Resources Division, Research Center for Chemistry, Biology, and Agriculture (CPQBA), State University of Campinas - UNICAMP, Paulínia, SP CEP 13081-970, Brazil. Electronic address: vmaia@cpqba.unicamp.br.
Microbiol Res ; 265: 127197, 2022 Dec.
Article en En | MEDLINE | ID: mdl-36174355
Antarctica is the coldest and driest continent on Earth, characterized by polyextreme environmental conditions, where species adapted form complex networks of interactions. Microbial communities growing in these harsh environments can form biofilms that help the associated species to survive and thrive. A rich body of knowledge describes environmental biofilm communities; however, most studies have focused on dominant community members rather than functional complexity and metabolic potential. To overcome these limitations, the present study used genome-centric metagenomics to describe two biofilm samples subjected to different temperature collected in Deception Island, Maritime Antarctica. The results unraveled a complex biofilm microbiome represented by 180 metagenome-assembled genomes. The potential metabolic interactions were investigated using metabolic flux balance analysis and revealed that purple bacteria are the community members with the highest correlations with other bacteria. Due to their predicted mixotrophic behavior, they may play a crucial role in the microbiome, likely supporting the heterotrophic species in biofilms. Metatranscriptomics results revealed that the chaperone system and proteins counteracting ROS and toxic compounds have a major role in maintaining bacterial cell homeostasis in sediments of volcanic origin.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Metagenómica / Microbiota Idioma: En Revista: Microbiol Res Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2022 Tipo del documento: Article Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Metagenómica / Microbiota Idioma: En Revista: Microbiol Res Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2022 Tipo del documento: Article Pais de publicación: Alemania