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FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis.
Sun, Bin; Bhati, Kaushal Kumar; Song, Peizhe; Edwards, Ashleigh; Petri, Louise; Kruusvee, Valdeko; Blaakmeer, Anko; Dolde, Ulla; Rodrigues, Vandasue; Straub, Daniel; Yang, Junbo; Jia, Guifang; Wenkel, Stephan.
Afiliación
  • Sun B; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
  • Bhati KK; Copenhagen Plant Science Centre, University of Copenhagen, Frederiksberg, Denmark.
  • Song P; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
  • Edwards A; Copenhagen Plant Science Centre, University of Copenhagen, Frederiksberg, Denmark.
  • Petri L; Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
  • Kruusvee V; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
  • Blaakmeer A; Copenhagen Plant Science Centre, University of Copenhagen, Frederiksberg, Denmark.
  • Dolde U; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
  • Rodrigues V; Copenhagen Plant Science Centre, University of Copenhagen, Frederiksberg, Denmark.
  • Straub D; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
  • Yang J; Copenhagen Plant Science Centre, University of Copenhagen, Frederiksberg, Denmark.
  • Jia G; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
  • Wenkel S; Copenhagen Plant Science Centre, University of Copenhagen, Frederiksberg, Denmark.
PLoS Genet ; 18(9): e1010386, 2022 09.
Article en En | MEDLINE | ID: mdl-36166469
Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3'-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3'-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: PLoS Genet Asunto de la revista: GENETICA Año: 2022 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: PLoS Genet Asunto de la revista: GENETICA Año: 2022 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos