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Gold(III) Complexes with Phenanthroline-derivatives Ligands Induce Apoptosis in Human Colorectal and Breast Cancer Cell Lines.
Milutinovic, Milena G; Milivojevic, Nevena N; Dordevic, Nevena M; Nikodijevic, Danijela D; Radisavljevic, Snezana R; Dekovic Kesic, Ana S; Markovic, Snezana D.
Afiliación
  • Milutinovic MG; University of Kragujevac, Department of Biology and Ecology, Faculty of Science, Radoja Domanovica 12, 34000 Kragujevac, Serbia. Electronic address: milena.milutinovic@pmf.kg.ac.rs.
  • Milivojevic NN; University of Kragujevac, Institute for Information Technologies Kragujevac, Department of Natural Sciences, Jovana Cvijica bb, 34000 Kragujevac, Serbia.
  • Dordevic NM; University of Kragujevac, Department of Biology and Ecology, Faculty of Science, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
  • Nikodijevic DD; University of Kragujevac, Department of Biology and Ecology, Faculty of Science, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
  • Radisavljevic SR; University of Kragujevac, Department of Chemistry, Faculty of Science, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
  • Dekovic Kesic AS; University of Kragujevac, Institute for Information Technologies Kragujevac, Department of Natural Sciences, Jovana Cvijica bb, 34000 Kragujevac, Serbia.
  • Markovic SD; University of Kragujevac, Department of Biology and Ecology, Faculty of Science, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
J Pharm Sci ; 111(12): 3215-3223, 2022 12.
Article en En | MEDLINE | ID: mdl-36162493
Due to their promising effects, gold(III) complexes recently drew increasing attention in the design of new metal-based anticancer therapeutics. Two gold(III) complexes, square-planar [Au(DPP)Cl2]+ - Complex 1 and distorted square-pyramidal [Au(DMP)Cl3] - Complex 2 (where DPP=4,7-diphenyl-1,10-phenanthroline and DMP=2,9-dimethyl-1,10-phenanthroline) were previously synthetized, described and approved as complexes with pronounced cytotoxic effects on colorectal HCT-116 and breast MDA-MB-231 cancer cells. This study investigated the type of cell death by AO/EB double staining, and identification of possible targets responsible for their cytotoxicity, monitored by immunofluorescence and qPCR methods. Both complexes induced apoptosis in all applied concentrations. In the HCT-116 cells apoptosis was activated by external apoptotic pathway, via increase of Fas receptor protein expression and Caspase 8 gene expression. Also, the mitochondrial pathway was triggered by affecting the Bcl-2 members of regulatory proteins and increased caspase 9 protein expression. In MDA-MB-231 cells, apoptosis was initiated from the mitochondria, due to disbalance between expressions of pro- and anti-apoptotic Bcl-2 family members and caspase 9 activation. Complex 1 shows better activity compared to Complex 2, which is in accordance with its structural characteristics. The results deal weighty data about proapoptotic activity of gold(III) complexes and highlighted potential targets for cancer therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Neoplasias Colorrectales Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: J Pharm Sci Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Neoplasias Colorrectales Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: J Pharm Sci Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos