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The protective effect of low-dose minocycline on brain microvascular ultrastructure in a rodent model of subarachnoid hemorrhage.
Gendosz de Carrillo, Daria; Student, Sebastian; Bula, Daniel; Mielanczyk, Lukasz; Burek, Malgorzata; Meybohm, Patrick; Jedrzejowska-Szypulka, Halina.
Afiliación
  • Gendosz de Carrillo D; Department of Physiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Poniatowskiego 15, 40-055, Katowice, Poland. dariagendosz@gmail.com.
  • Student S; Department of Histology and Cell Pathology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Poniatowskiego 15, 40-055, Katowice, Poland. dariagendosz@gmail.com.
  • Bula D; Department of Systems Biology and Engineering, Silesian University of Technology, Gliwice, Poland.
  • Mielanczyk L; Biotechnology Centre, Silesian University of Technology, Gliwice, Poland.
  • Burek M; Oncological and Reconstructive Surgery Department, Gliwice Branch, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice, Poland.
  • Meybohm P; Department of Histology and Cell Pathology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Poniatowskiego 15, 40-055, Katowice, Poland.
  • Jedrzejowska-Szypulka H; Department of Anaesthesiology, Intensive Care, Emergency and Pain Medicine, University Hospital Würzburg, Würzburg, Germany.
Histochem Cell Biol ; 159(1): 91-114, 2023 Jan.
Article en En | MEDLINE | ID: mdl-36153470
The multifaceted nature of subarachnoid hemorrhage (SAH) pathogenesis is poorly understood. To date, no pharmacological agent has been found to be efficacious for the prevention of brain injury when used for acute SAH intervention. This study was undertaken to evaluate the beneficial effects of low-dose neuroprotective agent minocycline on brain microvascular ultrastructures that have not been studied in detail. We studied SAH brain injury using an in vivo prechiasmatic subarachnoid hemorrhage rodent model. We analyzed the qualitative and quantitative ultrastructural morphology of capillaries and surrounding neuropil in the rodent brains with SAH and/or minocycline administration. Here, we report that low-dose minocycline (1 mg/kg) displayed protective effects on capillaries and surrounding cells from significant SAH-induced changes. Ultrastructural morphology analysis revealed also that minocycline stopped endothelial cells from abnormal production of vacuoles and vesicles that compromise blood-brain barrier (BBB) transcellular transport. The reported ultrastructural abnormalities as well as neuroprotective effects of minocycline during SAH were not directly mediated by inhibition of MMP-2, MMP-9, or EMMPRIN. However, SAH brain tissue treated with minocycline was protected from development of other morphological features associated with oxidative stress and the presence of immune cells in the perivascular space. These data advance the knowledge on the effect of SAH on brain tissue ultrastructure in an SAH rodent model and the neuroprotective effect of minocycline when administered in low doses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemorragia Subaracnoidea / Lesiones Encefálicas / Fármacos Neuroprotectores Tipo de estudio: Qualitative_research Límite: Animals Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemorragia Subaracnoidea / Lesiones Encefálicas / Fármacos Neuroprotectores Tipo de estudio: Qualitative_research Límite: Animals Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania