Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues.

Martinek, Jan; Wu, Te-Chia; Sun, Lili; Lin, Jianan; Kim, Kyung In; Marches, Florentina; Robson, Paul; George, Joshy; Palucka, Karolina

Martinek, Jan; Wu, Te-Chia; Sun, Lili; Lin, Jianan; Kim, Kyung In; Marches, Florentina; Robson, Paul; George, Joshy; Palucka, Karolina.

Afiliación

Martinek J; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA. Electronic address: jan.martinek@jax.org.
Wu TC; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Sun L; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Lin J; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Kim KI; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Marches F; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Robson P; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA; Department of Genetics and Genome Sciences, UConn Health, Farmington, CT, USA.
George J; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Palucka K; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA; Department of Genetics and Genome Sciences, UConn Health, Farmington, CT, USA. Electronic address: karolina.palucka@jax.org.

STAR Protoc ; 3(4): 101698, 2022 12 16.

Article en En | MEDLINE | ID: mdl-36149794

Asunto(s)

Captura por Microdisección con Láser; Melanoma; Animales; Humanos; Ratones; Técnica del Anticuerpo Fluorescente; Perfilación de la Expresión Génica/métodos; Captura por Microdisección con Láser/métodos; Melanoma/genética; Melanoma/cirugía; Transcriptoma/genética

Palabras clave

Cancer; Cell isolation; Immunology; Microscopy; RNAseq; Single cell

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Captura por Microdisección con Láser / Melanoma Límite: Animals / Humans Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos

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