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Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model.
Gargaté, Maria João; Vilares, Anabela; Ferreira, Idalina; Reis, Tânia; Martins, Susana; Mendonça, Joana; Borges, Vítor; Gomes, João Paulo.
Afiliación
  • Gargaté MJ; National Reference Laboratory of Parasitic and Fungal Infections, Department of Infectious Diseases, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
  • Vilares A; National Reference Laboratory of Parasitic and Fungal Infections, Department of Infectious Diseases, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
  • Ferreira I; National Reference Laboratory of Parasitic and Fungal Infections, Department of Infectious Diseases, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
  • Reis T; National Reference Laboratory of Parasitic and Fungal Infections, Department of Infectious Diseases, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
  • Martins S; National Reference Laboratory of Parasitic and Fungal Infections, Department of Infectious Diseases, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
  • Mendonça J; Innovation and Technology Unit, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
  • Borges V; Genomics and Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
  • Gomes JP; Genomics and Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health Doctor Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisbon, Portugal.
Pathogens ; 11(9)2022 Sep 13.
Article en En | MEDLINE | ID: mdl-36145470
Toxoplasma gondii is an obligate intracellular protozoan. In pregnant women, it can lead to severe birth defects or intrauterine death of the fetus. Most of what is currently know on cell biology of T. gondii comes from studies relying on the RH strain propagated in mice. According to the recommendations concerning the animal welfare, we assayed in vitro/in vivo procedures to replace, or at least reduce, the demanding animal model for strain propagation. We evaluated the genetic and phenotypic stability of the RH strain throughout its parallel continuous propagation in mice, in human foreskin fibroblasts (HFF) and in an alternate fashion of these two procedures. We also assessed the virulence impact on the RH strain after different periods of its long-term propagation strictly in cells. The RH strain completely lost its virulence after long-term passage in HFF. Nevertheless, we obtained a successful outcome with the alternate passaging of the parasite in HFF and in mice as this approach enabled T. gondii to maintain the evaluated phenotypic properties, mainly its virulence potential. Also, no genetic changes were observed in genes known to be highly polymorphic or involved in pathoadaptation. In conclusion, the alternate model seems to be a feasible method for T. gondii propagation and maintenance, strongly impacting the number of sacrificed mice.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pathogens Año: 2022 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pathogens Año: 2022 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza