Your browser doesn't support javascript.
loading
Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer.
Ding, Lin; Razavi Bazaz, Sajad; Shrestha, Jesus; A Amiri, Hoseyn; Mas-Hafi, Sima; Banerjee, Balarka; Vesey, Graham; Miansari, Morteza; Ebrahimi Warkiani, Majid.
Afiliación
  • Ding L; School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
  • Razavi Bazaz S; School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
  • Shrestha J; School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
  • A Amiri H; Micro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, Iran.
  • Mas-Hafi S; Cell Science Research Center, Department of Cancer Medicine, Royan Institute for Stem Cell Biology and Technology, Isar 11, Babol 47138-18983, Iran.
  • Banerjee B; Micro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, Iran.
  • Vesey G; Cell Science Research Center, Department of Cancer Medicine, Royan Institute for Stem Cell Biology and Technology, Isar 11, Babol 47138-18983, Iran.
  • Miansari M; Regeneus Ltd., Paddington, Sydney, NSW 2021, Australia.
  • Ebrahimi Warkiani M; Regeneus Ltd., Paddington, Sydney, NSW 2021, Australia.
Micromachines (Basel) ; 13(9)2022 Sep 13.
Article en En | MEDLINE | ID: mdl-36144139
Cryopreservation is the final step of stem cell production before the cryostorage of the product. Conventional methods of adding cryoprotecting agents (CPA) into the cells can be manual or automated with robotic arms. However, challenging issues with these methods at industrial-scale production are the insufficient mixing of cells and CPA, leading to damage of cells, discontinuous feeding, the batch-to-batch difference in products, and, occasionally, cross-contamination. Therefore, the current study proposes an alternative way to overcome the abovementioned challenges; a highly efficient micromixer for low-cost, continuous, labour-free, and automated mixing of stem cells with CPA solutions. Our results show that our micromixer provides a more homogenous mixing of cells and CPA compared to the manual mixing method, while the cell properties, including surface markers, differentiation potential, proliferation, morphology, and therapeutic potential, are well preserved.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Micromachines (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Micromachines (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza