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Novel Megaplasmid Driving NDM-1-Mediated Carbapenem Resistance in Klebsiella pneumoniae ST1588 in South America.
Quezada-Aguiluz, Mario; Opazo-Capurro, Andrés; Lincopan, Nilton; Esposito, Fernanda; Fuga, Bruna; Mella-Montecino, Sergio; Riedel, Gisela; Lima, Celia A; Bello-Toledo, Helia; Cifuentes, Marcela; Silva-Ojeda, Francisco; Barrera, Boris; Hormazábal, Juan C; González-Rocha, Gerardo.
Afiliación
  • Quezada-Aguiluz M; Laboratorio de Investigación en Agentes Antibacterianos (LIAA-UdeC), Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile.
  • Opazo-Capurro A; Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción 4030000, Chile.
  • Lincopan N; Millennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 8320000, Chile.
  • Esposito F; Centro Regional de Telemedicina y Telesalud del Biobío (CRT Biobío), Concepción 4030000, Chile.
  • Fuga B; Laboratorio de Investigación en Agentes Antibacterianos (LIAA-UdeC), Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile.
  • Mella-Montecino S; Millennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 8320000, Chile.
  • Riedel G; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo 05508-000, Brazil.
  • Lima CA; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
  • Bello-Toledo H; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo 05508-000, Brazil.
  • Cifuentes M; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo 05508-000, Brazil.
  • Silva-Ojeda F; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
  • Barrera B; Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción 4030000, Chile.
  • Hormazábal JC; Unidad de Infectología, Hospital Regional "Dr. Guillermo Grant Benavente", Concepción 4030000, Chile.
  • González-Rocha G; Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción 4030000, Chile.
Antibiotics (Basel) ; 11(9)2022 Sep 07.
Article en En | MEDLINE | ID: mdl-36139987
Carbapenem-resistant Enterobacterales (CRE) is a critical public health problem in South America, where the prevalence of NDM metallo-betalactamases has increased substantially in recent years. In this study, we used whole genome sequencing to characterize a multidrug-resistant (MDR) Klebsiella pneumoniae (UCO-361 strain) clinical isolate from a teaching hospital in Chile. Using long-read (Nanopore) and short-read (Illumina) sequence data, we identified a novel un-typeable megaplasmid (314,976 kb, pNDM-1_UCO-361) carrying the blaNDM-1 carbapenem resistance gene within a Tn3000 transposon. Strikingly, conjugal transfer of pNDM-1_UCO-361 plasmid only occurs at low temperatures with a high frequency of 4.3 × 10-6 transconjugants/receptors at 27 °C. UCO-361 belonged to the ST1588 clone, previously identified in Latin America, and harbored aminoglycoside, extended-spectrum ß-lactamases (ESBLs), carbapenem, and quinolone-resistance determinants. These findings suggest that blaNDM-1-bearing megaplasmids can be adapted to carriage by some K. pneumoniae lineages, whereas its conjugation at low temperatures could contribute to rapid dissemination at the human-environmental interface.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Antibiotics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Antibiotics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza