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A Toolkit for Engineering Proteins in Living Cells: Peptide with a Tryptophan-Selective Ru-TAP Complex to Regioselectively Photolabel Specific Proteins.
Chen, Tzu-Ho; Garnir, Kevin; Chen, Chong-Yan; Jian, Cheng-Bang; Gao, Hua-De; Cheng, Bill; Tseng, Mei-Chun; Moucheron, Cécile; Kirsch-De Mesmaeker, Andrée; Lee, Hsien-Ming.
Afiliación
  • Chen TH; Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan.
  • Garnir K; Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
  • Chen CY; Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan.
  • Jian CB; Laboratoire de Chimie Organique et Photochimie CP160/08, Université libre de Bruxelles, 50 Av. Franklin D. Roosevelt, 1050 Brussels, Belgium.
  • Gao HD; Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan.
  • Cheng B; Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
  • Tseng MC; Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan.
  • Moucheron C; Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan.
  • Kirsch-De Mesmaeker A; Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
  • Lee HM; Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan.
J Am Chem Soc ; 144(39): 18117-18125, 2022 10 05.
Article en En | MEDLINE | ID: mdl-36135325
Using a chemical approach to crosslink functionally versatile bioeffectors (such as peptides) to native proteins of interest (POI) directly inside a living cell is a useful toolbox for chemical biologists. However, this goal has not been reached due to unsatisfactory chemoselectivity, regioselectivity, and protein selectivity in protein labeling within living cells. Herein, we report the proof of concept of a cytocompatible and highly selective photolabeling strategy using a tryptophan-specific Ru-TAP complex as a photocrosslinker. Aside from the high selectivity, the photolabeling is blue light-driven by a photoinduced electron transfer (PeT) and allows the bioeffector to bear an additional UV-responsive unit. The two different photosensitivities are demonstrated by blue light-photocrosslinking a UV-sensitive peptide to POI. Our visible light photolabeling can generate photocaged proteins for subsequent activity manipulation by UV light. Cytoskeletal dynamics regulation is demonstrated in living cells via the unprecedented POI photomanipulation and proves that our methodology opens a new avenue to endogenous protein modification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triptófano / Proteínas Idioma: En Revista: J Am Chem Soc Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triptófano / Proteínas Idioma: En Revista: J Am Chem Soc Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos