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MicroRNA-263b confers imidacloprid resistance in Sitobion miscanthi (Takahashi) by regulating the expression of the nAChRß1 subunit.
Zhang, Bai-Zhong; Zhang, Meng-Yuan; Li, Ya-She; Hu, Gui-Lei; Fan, Xin-Zheng; Guo, Tian-Xin; Zhou, Feng; Zhang, Pei; Wu, Yan-Bing; Gao, Yang-Fan; Gao, Xi-Wu.
Afiliación
  • Zhang BZ; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Zhang MY; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Li YS; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Hu GL; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Fan XZ; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Guo TX; Department of Entomology, China Agricultural University, Beijing 100193, PR China.
  • Zhou F; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Zhang P; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Wu YB; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Gao YF; College of Resources and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
  • Gao XW; Department of Entomology, China Agricultural University, Beijing 100193, PR China. Electronic address: gaoxiwu@263.net.cn.
Pestic Biochem Physiol ; 187: 105218, 2022 Oct.
Article en En | MEDLINE | ID: mdl-36127060
The Chinese wheat aphid Sitobion miscanthi (CWA) is an important harmful pest in wheat fields. Imidacloprid plays a critical role in controlling pests with sucking mouthparts. However, imidacloprid-resistant pests have been observed after insecticide overuse. Point mutations and low expression levels of the nicotinic acetylcholine receptor ß1 (nAchRß1) subunit are the main imidacloprid-resistant mechanisms. However, the regulatory mechanism underlying nAChRß1 subunit expression is poorly understood. In this study, a target of miR-263b was isolated from the 5'UTR of the nAchRß1 subunit in the CWA. Low expression levels were found in the imidacloprid-resistant strain CWA. Luciferase reporter assays showed that miR-263b could combine with the 5'UTR of the nAChRß1 subunit and suppress its expression by binding to a site in the CWA. Aphids treated with the miR-263b agomir exhibited a significantly reduced abundance of the nAchRß1 subunit and increased imidacloprid resistance. In contrast, aphids treated with the miR-263b antagomir exhibited significantly increased nAchRß1 subunit abundance and decreased imidacloprid resistance. These results provide a basis for an improved understanding of the posttranscriptional regulatory mechanism of the nAChRß1 subunit and further elucidate the function of miRNAs in regulating susceptibility to imidacloprid in the CWA. These results provide a better understanding of the mechanisms of posttranscriptional regulation of nAChRß1 and will be helpful for further studies on the role of miRNAs in the regulation of nAChRß1 subunit resistance in homopteran pests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Áfidos / Receptores Nicotínicos / MicroARNs / Insecticidas Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Áfidos / Receptores Nicotínicos / MicroARNs / Insecticidas Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos