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Acquisition of hypoxia inducibility by oxygen sensing N-terminal cysteine oxidase in spermatophytes.
Weits, Daan A; Zhou, Lina; Giuntoli, Beatrice; Carbonare, Laura Dalle; Iacopino, Sergio; Piccinini, Luca; Lombardi, Lara; Shukla, Vinay; Bui, Liem T; Novi, Giacomo; van Dongen, Joost T; Licausi, Francesco.
Afiliación
  • Weits DA; Institute of Biology 1, Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
  • Zhou L; Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.
  • Giuntoli B; Plant-Environment Signaling, Institute of Environmental Biology, Utrecht University, Utrecht, The Netherlands.
  • Carbonare LD; Institute of Biology 1, Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
  • Iacopino S; School of Life Sciences, Lanzhou University, Lanzhou, China.
  • Piccinini L; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an, China.
  • Lombardi L; Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.
  • Shukla V; Department of Biology, University of Pisa, Pisa, Italy.
  • Bui LT; Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.
  • Novi G; Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.
  • van Dongen JT; Department of Biology, University of Pisa, Pisa, Italy.
  • Licausi F; Department of Plant Sciences, University of Oxford, Oxford, UK.
Plant Cell Environ ; 46(1): 322-338, 2023 01.
Article en En | MEDLINE | ID: mdl-36120894
N-terminal cysteine oxidases (NCOs) use molecular oxygen to oxidise the amino-terminal cysteine of specific proteins, thereby initiating the proteolytic N-degron pathway. To expand the characterisation of the plant family of NCOs (plant cysteine oxidases [PCOs]), we performed a phylogenetic analysis across different taxa in terms of sequence similarity and transcriptional regulation. Based on this survey, we propose a distinction of PCOs into two main groups. A-type PCOs are conserved across all plant species and are generally unaffected at the messenger RNA level by oxygen availability. Instead, B-type PCOs appeared in spermatophytes to acquire transcriptional regulation in response to hypoxia. The inactivation of two A-type PCOs in Arabidopsis thaliana, PCO4 and PCO5, is sufficient to activate the anaerobic response in young seedlings, whereas the additional removal of B-type PCOs leads to a stronger induction of anaerobic genes and impairs plant growth and development. Our results show that both PCO types are required to regulate the anaerobic response in angiosperms. Therefore, while it is possible to distinguish two clades within the PCO family, we conclude that they all contribute to restrain the anaerobic transcriptional programme in normoxic conditions and together generate a molecular switch to toggle the hypoxic response.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Cisteína-Dioxigenasa Idioma: En Revista: Plant Cell Environ Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Cisteína-Dioxigenasa Idioma: En Revista: Plant Cell Environ Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos