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The application of the FLOTAC technique for detection of helminth eggs of medical and veterinary importance in soil samples.
do Nascimento Ramos, Ingrid Carla; Ramos, Rafael Antonio Nascimento; de Macedo, Lucia Oliveira; de Carvalho, Gílcia Aparecida; Alves, Leucio Câmara.
Afiliación
  • do Nascimento Ramos IC; Graduate Program of Animal Bioscience, Federal Rural University of Pernambuco, Recife, Brazil; Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil.
  • Ramos RAN; Laboratory of Parasitology, Federal University of the Agreste of Pernambuco, Garanhuns, Brazil. Electronic address: rafael.ramos@ufape.edu.br.
  • de Macedo LO; Graduate Program of Animal Bioscience, Federal Rural University of Pernambuco, Recife, Brazil; Laboratory of Parasitology, Federal University of the Agreste of Pernambuco, Garanhuns, Brazil.
  • de Carvalho GA; Laboratory of Parasitology, Federal University of the Agreste of Pernambuco, Garanhuns, Brazil.
  • Alves LC; Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil.
Exp Parasitol ; 242: 108379, 2022 Nov.
Article en En | MEDLINE | ID: mdl-36116521
The FLOTAC technique was initially developed to detected infective stages of parasites in animal and human feces. Recently, its applicability has been extended by the use in pastures and vegetables for human consumption. However, its use for the detection of parasites in the soil has never been investigated. In this study, we assessed the performance of the FLOTAC and compared with centrifugal flotation (CFT) and spontaneous sedimentation (SST) techniques. A total of 50 soil samples were collected from the Metropolitan region of Recife, Northeastern Brazil. Initially, samples were standardized, and then assessed in duplicate by the FLOTAC, CFT and SST. Cohen's kappa coefficient, sensitivity, specificity, positive and negative predictive values, and accuracy of the FLOTAC, CFT and SST were assessed using the results of the combination of all three techniques as gold standard. Out of all analyses performed, in 96% (48/50), helminth eggs of parasites were detected. In particular, 96% (48/50) of samples analysed through the FLOTAC technique, 76% (38/50) for CFT and 28% (14/50) for SST were positive. Nematode eggs of the Ancylostomatidae and Ascarididae families were those mostly detected in this study. Overall, the FLOTAC presented high values of sensitivity, demonstrating that this method may also be employed for detection of parasites in soil samples. This study opens new possibilities for the use of the FLOTAC technique, highlighting its role as a potential tool for detecting environmental contamination by parasites of medical and veterinary importance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parásitos / Helmintos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Exp Parasitol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parásitos / Helmintos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Exp Parasitol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos