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Activity, selection response and molecular mode of action of the isoxazoline afoxolaner in Tetranychus urticae.
Mermans, Catherine; Dermauw, Wannes; Geibel, Sven; Van Leeuwen, Thomas.
Afiliación
  • Mermans C; Department of Plants and Crops | Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
  • Dermauw W; Department of Plants and Crops | Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
  • Geibel S; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, Belgium.
  • Van Leeuwen T; CropScience Division, Bayer AG, Monheim, Germany.
Pest Manag Sci ; 79(1): 183-193, 2023 Jan.
Article en En | MEDLINE | ID: mdl-36116012
BACKGROUND: Afoxolaner is a novel representative of the isoxazolines, a class of ectoparasiticides which has been commercialized for the control of tick and flea infestations in dogs. In this study, the biological efficacy of afoxolaner against the two-spotted spider mite Tetranychus urticae was evaluated. Furthermore, as isoxazolines are known inhibitors of γ-aminobutyric acid-gated chloride channels (GABACls), the molecular mode of action of afoxolaner on T. urticae GABACls (TuRdls) was studied using functional expression in Xenopus oocytes followed by two-electrode voltage-clamp (TEVC) electrophysiology, and results were compared with inhibition by fluralaner, fipronil and endosulfan. To examine the influence of known GABACl resistance mutations, H301A, I305T and A350T substitutions in TuRdl1 and a S301A substitution in TuRdl2 were introduced. RESULTS: Bioasassays revealed excellent efficacy of afoxolaner against all developmental stages and no cross-resistance was found in a panel of strains resistant to most currently used acaricides. Laboratory selection over a period of 3 years did not result in resistance. TEVC revealed clear antagonistic activity of afoxolaner and fluralaner for all homomeric TuRdl1/2/3 channels. The introduction of single, double or triple mutations to TuRdl1 and TuRdl2 did not lower channel sensitivity. By contrast, both endosulfan and fipronil had minimal antagonistic activities against TuRdl1/2/3, and channels carrying single mutations, whereas the sensitivity of double and triple TuRdl1 mutants was significantly increased. CONCLUSIONS: Our results demonstrate that afoxolaner is a potent antagonist of GABACls of T. urticae and has a powerful mode of action to control spider mites. © 2022 Society of Chemical Industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Límite: Animals Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Límite: Animals Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido